1. Introduction to ‘Inflammation’
Dr. habil. Kőhidai László
Assoc. Professor
Dept. Genetics, Cell- and Immunobiology
Semelweis University
Chemotaxis - Elective Course
2010/2011 – EM-ED

2. Components of inflammatory response
Cells of
connective tissue
Vascular lumen
Extracellular matrix
Collagen IV.
Laminin
Fibronectin
Elastin
Proteoglycan

3. Inflammation phase 1 contraction of endothel histamine, bradykinin

4. Inflammation phase 2
injury of endothel
bacterial, burns
2-12 hrs

5. Inflammation phase 3 accumulation of leukocytes
release of proteolytic enzymes

6. Inflammation phase 4
regeneration of capillaries

7. Activation of endothel
PAF
P selectin
Cytokine NO
IL-8
E selectin
PAF = platelet activating factor

8. Most significant groups of chemoattractant substances in inflammation
Soluble bacterial products (e.g. fMLF)
Products of complemet cascade (e.g. C5a)
Arachidonic acid metabolism –
products of lipoxigenase line (LTB4)

9. Ag-Ab complex C1 C3
C4+C2 B
C1a
C3b D
C4bC2a C3
C3bBb
C4bC2aC3b
C3a
C3b C5
C3bBb3b
C5a
C5b
Alternative
pathway
Classical pathway
C5-9
Membrane attack complex

10. Plasma origin chemoattractant substances
Complement
C3a
C4a
C5a
C5a desArg
Fibrinogen
fibrinopeptide B D E

11. Arachidonic acid metabolites
Phospholipides of surface membrane
Phospholipases
ARACHIDONIC ACID
lipoxigenase
cyclooxygenase
Leukotrien A4 (LTA4)
Prostaglandin G2 (PGG2)
PGH2
PGD2
PGE2
PGF2a
LTC4
LTD4
LTE4
LTB4

12. Characterization of cytokines
small molecular mass, glycisilated proteins
members of inflammatory and immune processes
short lifespan
auto- and paracrine actions
significant effects even in low concentrations
inducers of RNA and protein synthesis in the target cells
frequent inducer and blocker crossreactions
transmodulation on receptors of each other

13. IL-1 TNF Producer and target cells of cytokines granulocyte lymphocyte
macrophage
keratinocyte
astrocyte
mezangial cell
macrophage
NK-cell
mast cell
IL-1
TNF
granulocyte
lymphocyte +
vascular responses

14. IL-1 TNF IL-8 synthesis Inducer crossreactions between cytokines
monocyte
macrophage
fibroblast
endothel
epithel
IL-8
synthesis
Inducer
crossreactions
between
cytokines

15. Neutrophil-dependent
C5a M
TNF
IL-1 U U U
Endothel-dependent

16. Diversity of dynamism in migration of different cells24 48
(hrs)
monocytes
neutrophils
cell number
Acute phase