1. Enzyme Action Enzymes … proteins with catalytic function
… possess 1O, 2O, 3O and 4O structure
… perform a variety of reactions
e.g., hydrolysis, hydroxylation,
oxidation, reduction, transfer of groups …
… increase the rate of reaction (up to 1017-fold)
MEDC 527 Fall 2008

2. Enzyme Action Three dimensional structure of an enzyme
MEDC 527 Fall 2008

3. Enzyme Action Catalytic Triad Catalytic Triad
Chymotrypsin Acetylcholine Esterase
MEDC 527 Fall 2008

4. Chemical Mechanism of Enzyme Action
MEDC 527 Fall 2008

5. Michaelis – Menten Kinetics and Enzyme Inhibition
MEDC 527 Fall 2008