Presentation is loading. Please wait.

Presentation is loading. Please wait.

COL1A1-PDGFB Fusion Transcripts in Fibrosarcomatous Areas of Six Dermatofibrosarcomas Protuberans  Jian Wang, Yosuke Morimitsu, Sumika Okamoto, Masanori.

Published byGeoffrey Duval Modified over 6 years ago

Similar presentations

Presentation on theme: "COL1A1-PDGFB Fusion Transcripts in Fibrosarcomatous Areas of Six Dermatofibrosarcomas Protuberans  Jian Wang, Yosuke Morimitsu, Sumika Okamoto, Masanori."— Presentation transcript:

1 COL1A1-PDGFB Fusion Transcripts in Fibrosarcomatous Areas of Six Dermatofibrosarcomas Protuberans 
Jian Wang, Yosuke Morimitsu, Sumika Okamoto, Masanori Hisaoka, Tsuyoshi Ishida, Weiqi Sheng, Hiroshi Hashimoto  The Journal of Molecular Diagnostics  Volume 2, Issue 1, Pages (February 2000) DOI: /S (10) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Photomicrographs of dermatofibrosarcoma protuberans with fibrosarcomatous areas before (A) and after microdissection (B) in case 1.Asterisk represents a fibrosarcomatous area;triangle represents a conventional DFSP area (H&E; original magnification, ×2.5). The Journal of Molecular Diagnostics 2000 2, 47-52DOI: ( /S (10) ) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Histological appearance of DFSP-FS. A: Distinct storiform pattern in a conventional DFSP area with characteristic honeycomb infiltration of the adjacent fat tissue (case 4; H&E; original magnification, ×25).B: Herringbone structure in a fibrosarcomatous area of DFSP-FS (case 4; H&E; original magnification, ×50). The Journal of Molecular Diagnostics 2000 2, 47-52DOI: ( /S (10) ) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Expression of COL1A1-PDGFB fusion transcripts (A) and PBGD gene mRNA (housekeeping gene;B) in each sample of DFSP-FS. The number of the lane is identical to the case number in Table 2. The double bands in lane 5D were likely to be formed by DNA conformation changes, because the DNA sequences of the PCR products in both upper and lower bands in lane 5D were confirmed to be identical by the sequence analysis. M, 100-bp DNA ladder; D, conventional DFSP areas; F, fibrosarcomatous areas; N, negative control. The Journal of Molecular Diagnostics 2000 2, 47-52DOI: ( /S (10) ) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

5 Figure 4 Nucleotide sequence analysis of PCR products showing three types of COL1A1-PDGFB fusion transcripts composed of junctions between PDGFB exon 2 and COL1A1 exon 25 (A), exon 32 (B), or exon 46 (C). The Journal of Molecular Diagnostics 2000 2, 47-52DOI: ( /S (10) ) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Download ppt "COL1A1-PDGFB Fusion Transcripts in Fibrosarcomatous Areas of Six Dermatofibrosarcomas Protuberans  Jian Wang, Yosuke Morimitsu, Sumika Okamoto, Masanori."

Similar presentations

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,
CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.
Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,

Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,
Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.
High-Quality Genotyping Data from Formalin-Fixed, Paraffin-Embedded Tissue on the Drug Metabolizing Enzymes and Transporters Plus Array  Hanneke I. Vos,

High-Quality Genotyping Data from Formalin-Fixed, Paraffin-Embedded Tissue on the Drug Metabolizing Enzymes and Transporters Plus Array  Hanneke I. Vos,
Avery A. Sandberg, Julia A. Bridge  Cancer Genetics and Cytogenetics 

Avery A. Sandberg, Julia A. Bridge  Cancer Genetics and Cytogenetics 
Detection of Exon 12 Mutations in the JAK2 Gene

Detection of Exon 12 Mutations in the JAK2 Gene
Tumor Microsatellite Instability in Early Onset Gastric Cancer

Tumor Microsatellite Instability in Early Onset Gastric Cancer
Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers  Rebecca A. Betensky, Catherine.

Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers  Rebecca A. Betensky, Catherine.
Novel Functional Single Nucleotide Polymorphisms in the Latent Transforming Growth Factor-β Binding Protein-1L Promoter  Tomomi Higashi, Satoru Kyo, Masaki.

Novel Functional Single Nucleotide Polymorphisms in the Latent Transforming Growth Factor-β Binding Protein-1L Promoter  Tomomi Higashi, Satoru Kyo, Masaki.
Natalia Kholod, Jacques Boniver, Philippe Delvenne 

Natalia Kholod, Jacques Boniver, Philippe Delvenne 
Screening for Mutations in Kidney-Related Genes Using SURVEYOR Nuclease for Cleavage at Heteroduplex Mismatches  Konstantinos Voskarides, Constantinos.

Screening for Mutations in Kidney-Related Genes Using SURVEYOR Nuclease for Cleavage at Heteroduplex Mismatches  Konstantinos Voskarides, Constantinos.
Molecular Diagnosis of Necrotizing Fasciitis by 16S rRNA Gene Sequencing and Superantigen Gene Detection  Kenneth L. Muldrew, Jean F. Simpson, Charles.

Molecular Diagnosis of Necrotizing Fasciitis by 16S rRNA Gene Sequencing and Superantigen Gene Detection  Kenneth L. Muldrew, Jean F. Simpson, Charles.
Molecular Genetics of Pediatric Soft Tissue Tumors

Molecular Genetics of Pediatric Soft Tissue Tumors
Carmo Martins, Branca Cavaco, Giovanni Tonon, Frederic J

Carmo Martins, Branca Cavaco, Giovanni Tonon, Frederic J
Comprehensive Analysis of CBFβ-MYH11 Fusion Transcripts in Acute Myeloid Leukemia by RT-PCR Analysis  ShriHari S. Kadkol, Annette Bruno, Carol Dodge,

Comprehensive Analysis of CBFβ-MYH11 Fusion Transcripts in Acute Myeloid Leukemia by RT-PCR Analysis  ShriHari S. Kadkol, Annette Bruno, Carol Dodge,
Jonathan A. Schumacher, Stephen D. Jenson, Kojo S. J

Jonathan A. Schumacher, Stephen D. Jenson, Kojo S. J
Mutation Screening in Juvenile Polyposis Syndrome

Mutation Screening in Juvenile Polyposis Syndrome
IgH PCR of Zinc Formalin-Fixed, Paraffin-Embedded Non-Lymphomatous Gastric Samples Produces Artifactual “Clonal” Bands Not Observed in Paired Tissues.

IgH PCR of Zinc Formalin-Fixed, Paraffin-Embedded Non-Lymphomatous Gastric Samples Produces Artifactual “Clonal” Bands Not Observed in Paired Tissues.

Similar presentations

Ads by Google