1. miR-142-3p is a novel regulator of cell viability and proinflammatory signalling in endometrial stroma cells 
Christin Susanna Kästingschäfer, Sebastian Daniel Schäfer, Ludwig Kiesel, Martin Götte 
Reproductive BioMedicine Online 
Volume 30, Issue 5, Pages (May 2015)
DOI: /j.rbmo
Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions

2. Figure 1
Cells were transiently transfected with miRNA negative control, pre-miR-142-3p and anti-miR-142-3p (Schneider et al., 2013). (A) Steroid sulfatase mRNA expression in the endometrial stroma cell line St-T1b (fold change). Steroid sulfatase is downregulated 48 h after pre-miR-142-3p treatment, and upregulated after anti-miR-142-3p treatment. [*] P ≤ (B) gp130 mRNA expression in the endometrial stroma cell line St-T1b as well as primary endometrial stroma cells of six patients (fold change). gp130 mRNA expression is highly significantly [***] P ≤ 0.01 reduced 48 h after pre-miR-142-3p treatment. Gene expression was normalized to 18S rRNA and presented as mean values +/–SD. (C) Representative Western blotting of St-T1b cell extracts reveals reduction of gp130 protein expression 48 h after pre-miR-142-3p treatment. Lower panel shows densitometric quantification of three independent experiments. [*] P ≤ (D) mRNA expression of β-actin and BAK1 is not influenced by miR-142-3p upregulation or inhibition (qPCR) [n.s.]. (E) Western blotting analysis of interleukin-6 (Il-6) signal transduction using STAT3 phosphorylation as readout. Pre-miR-142-3p treatment reduced basal levels of phosphorylated STAT3 (IL-6 Ø) [n.s.], reduced the increase (IL-6 10 min) and accelerated the decrease (IL-6 30 min) of activation after IL-6 treatment. Lower panel shows densitometric quantification of three independent experiments. [*] P ≤ (F) Immunofluorescence staining of STAT3 30 min after interleukin-6 treatment. Nuclear translocation is absent after pre-miR-142-3p treatment. (G) Cell viability was measured by MTT assay 96 h after cell plating (625 cells / 100 µl). pre-miR-142-3p treatment significantly reduced cell viability. [***] P ≤ Values are presented as mean percentage of control +/–SD.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2015 Reproductive Healthcare Ltd. Terms and Conditions