1. Figure S1 A B C * * * * * * * * * * * * * * * * * * * * * * * * * * *
Mi-2 081
Mi-2 081
Mi-2 081 * * * * * * * * * * * * * * * * * * *
Fraction of inputt * * * *
Fraction of inputt * * *
Fraction of inputt *
Mi-2 082
Mi-2 082
Mi-2 082 * * * * * * * * * * * * * * * * * * * *
Fraction of inputt * *
Fraction of inputt * * * *
Fraction of inputt * *

2. Figure S2 A B C D * * * * * * * * * * * * * * * * * * * * * * * * * *
Fraction of inputt * * * * * * * * * * * C D * * * * * * * * * *
Fraction of inputt * * *

3. Figure S3 fetal liver megakaryocytes * * * * * * * *
0.01
0.02
0.03
0.04
0.05
0.06
0.07 * * * * * *
Fraction of inputt
gIgG
MTA2
RbAp46
Ib
Mpl
Gp9
Gp1b
Pf4
neg. Ctr
Hbb-b1
pro

4. Figure S4 A  B  % activity 5% input triple mut GST K5A WT  
MTA-1 
MTA-2 
RbAp48
RbAp46 
Coomassie B
140
120
100
% activity 80 60 40 20
GAL4 WT
K5A
triple mut

5. Figure S5 (-) Fold Activation 70 P=0.06 None GATA-1 FOG-1 GATA-1/FOG-160 50 40
Fold Activation 30 20 10
(-)
mock
siRNA ctr1
siRNA/MTA

6. Figure S6 A B Ctr. Agouti mice 1 2 3 4 5 6 8 7 9 10 M 13.5kb 11.4kb
WT Locus
Targeting
Vector
HSV-TK
5’ Arm, 12kb
3’ Arm, 5kb
FRT
exon 2
MSRRKQSNPRQIK
neo
probe
exon 1
EcoRV
13.5kb
11.4kb
R3GR4GK5A
(Sac1)
MSGGAQSNPRQIK
Targeted
neo excised
Locus A
Ctr.
Agouti mice 1 2 3 4 5 6 8 7 B 9 10 M
13.5kb
11.4kb

7. Figure S6 C D E MK Ery Ery FOG-1 ß-actin +/+ ki/ki +/+ ki/ki +/+ ki/kiwt
targeted
neo excised
PCR products:
208bp
> 2kb
278bp C
278bp
208bp D 1 2 3 4 5 6 8 7
100
200
300
400
500
600 M
Mice from +/ki and +/ki crosses E MK
Ery
Ery
FOG-1
ß-actin
+/+ ki/ki
+/+ ki/ki
+/+ ki/ki
FOG-1
FOG-1
ß-actin
ß-actin