4. Investigation of photoinduced reactivity of Glue-FITC:
1- Absorption spectra:
Glue-FITC 3µM + BSA 10µM
in Tris-HCl buffer (Tris – 20mM, pH- 7.0) +
UV irradiation ( nm band pass filter)
Observed decrease in the absorption intensity
at nm.
Due to the photochemical covalent binding of
benzophenone (BP) with protein.
Glue+ BSA
Glue
BSA

5. 2- Sodium dodecyl sulfate polyacrylamide gel
electrophoresis (SDS-PAGE)

6. 3- Kinesin/microtubule biomachinery system:
+ MT
+ MT +Glue-FITC +
UV irradiation
+ Glue
+ UV irradiation
Kinesin +
Cy5 labeled microtubules
Glue-FITC
UV irradiation.

7. BP is essential for the covalent immobilization:

8. Optical tweezers: To investigate the change in the binding strength between kinesin and the microtubules upon
adhesion with glue and subsequent photoclicking.
Bright field microscopy traces:

9. Conclusion:
Study demonstrated that water soluble GlueBP-FITC can serve as a photoclickable molecular
glue that covalently attaches to proteins.
As studied by “optical tweezers”, the temporal-to-permanent photochemical transformation
gives rise to a notable change in binding strength.
Protein-Glue study (with BSA and kinesin) shows that, both non covalent and covalent
interaction affects the secondary structure of the proteins or their biological functions.
Glue might be a potent candidate for the development of targeted cancer therapy.