1. Bacterial genetics

20. Hfr leu+ thr+pro+lac+bio+Sms X F- leu- thr-pro-lac-bio-SmR
What medium should we use to obtain
1. leu+ transconjugants
2. pro+ transconjugants
3. lac+ transconjugants

21. Hfr leu+ thr+pro+lac+bio+Sms X F- leu- thr-pro-lac-bio-SmR time
1. leu+ transconjugants
2. pro+ transconjugants
3. lac+ transconjugants
What are the map positions of
leu, pro, and lac?
time
No.of colonies on medium 1 2 3 5 10 8 15 28 6 20 48 16 25 68 26 11

25. Figure 9.30 25

26. Mobile Genetic Elements
IS elements transfer by one of 2 mechanisms:
- Replicative or nonreplicative transposition
Figure 9.31 26

27. Figure 9.9 27