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Single-Run, Parallel Detection of DNA from Three Pneumonia-Producing Bacteria by Real-Time Polymerase Chain Reaction  Reinhard B. Raggam, Eva Leitner,

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Presentation on theme: "Single-Run, Parallel Detection of DNA from Three Pneumonia-Producing Bacteria by Real-Time Polymerase Chain Reaction  Reinhard B. Raggam, Eva Leitner,"— Presentation transcript:

1 Single-Run, Parallel Detection of DNA from Three Pneumonia-Producing Bacteria by Real-Time Polymerase Chain Reaction  Reinhard B. Raggam, Eva Leitner, Jörg Berg, Gerhard Mühlbauer, Egon Marth, Harald H. Kessler  The Journal of Molecular Diagnostics  Volume 7, Issue 1, Pages (February 2005) DOI: /S (10) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 DNA fragment including the specific forward and reverse primer sequences of Chlamydia pneumoniae (CP)-, Legionella pneumophila (LP)-, and Mycoplasma pneumoniae (MP)-specific real-time PCRs. The center box represents the heterologous DNA derived from the neomycin phosphotransferase gene (neo), which is detected with one pair of fluorescence resonance energy transfer hybridization probes. The composite primers for the preparative PCRs are shown as horizontal lines from bottom up in the order of their use. Primer sequences are listed in Table 1. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Fluorescence versus cycle number plots of 10-fold dilutions of a bronchoalveolar lavage specimen spiked with standards of the Mycoplasma pneumoniae strain (5000, 500, 50, and 5 CFU per PCR reaction) and repeat tested showing Mycoplasma-specific amplification products (channel F2/backF1; a) and the corresponding internal control-specific amplification products (channel F3/backF1; b). The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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