1. Volume 138, Issue 7, Pages 2468-2476.e1 (June 2010)
The Hyperphagic Effect of Ghrelin Is Inhibited in Mice by a Diet High in Fat 
James V. Gardiner, Daniel Campbell, Michael Patterson, Aysha Kent, Mohammed A. Ghatei, Stephen R. Bloom, Gavin A. Bewick 
Gastroenterology 
Volume 138, Issue 7, Pages e1 (June 2010)
DOI: /j.gastro
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2. Figure 1
Excess ghrelin protects against diet-induced obesity by reducing food intake. Male mice (n = 6) were fed either RC or an HFD from 5 weeks of age. (A) Cumulative energy intake; (B) body weight curves; (C) body composition measured at 16 weeks of age; (D) plasma leptin concentration measured at 16 weeks of age; (E) cumulative food intake measured by the comprehensive laboratory animal monitoring system (CLAMS); (F) average light and dark phase food intake measured by the CLAMS. Data represent mean ± standard error of mean. *P < .05 compared with TG HF.
Gastroenterology  , e1DOI: ( /j.gastro )
Copyright © 2010 AGA Institute Terms and Conditions

3. Figure 2
High-fat feeding does not exacerbate ghrelin-induced impairment of glucose tolerance. Male mice (n = 6) were fed either RC or HFD from 5 weeks of age. (A) Fasting plasma glucose; (B) fasting insulin levels measured at 16 weeks of age; (C) plasma glucose disposal during a glucose tolerance test at 14 weeks of age; (D) area under the curve for C *P < .05 compared with RC Wt; (E) plasma glucose during an insulin tolerance test at 15 weeks of age; (F) area under the curve for E. Data represent mean ± standard error of mean. *P < .05.
Gastroenterology  , e1DOI: ( /j.gastro )
Copyright © 2010 AGA Institute Terms and Conditions

4. Figure 3
Food preference, hypothalamic neuropeptide expression, and acylation substrate are unchanged by high-fat feeding. (A) Average daily food intake in male mice (n = 8) fed a choice of RC and HFD from 5 weeks of age; (B) proopiomelanocortin (POMC) and neuropeptide Y (NPY) messenger RNA expression in whole hypothalamus extracts from male mice (n = 6) fed an HFD. Acylated species of ghrelin in Wt (C and D) and Tg (E and F) plasma from mice fed RC HFD resolved using FPLC. Data represent mean ± standard error of mean. *P < .05.
Gastroenterology  , e1DOI: ( /j.gastro )
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5. Figure 4
Chronic administration of ghrelin does not increase food intake under high-fat feeding conditions. Male mice (n = 8) were implanted with subcutaneous osmotic minipumps, which delivered 0.6 nmol/g/day of ghrelin or saline intraperotoneally. Mice were fed either regular or HFD. (A) Plasma active ghrelin (*P < .05); (B) body weight; (C) cumulative food intake; (D) cumulative energy intake. Data represent mean ± standard error of mean. (*P < .05 between saline and ghrelin-treated mice fed regular chow.)
Gastroenterology  , e1DOI: ( /j.gastro )
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6. Supplementary Figure 1
A circulating acylated ghrelin measured in male mice fed an HFD n = 9. Male mice n = 6 were fed either RC or an HFD from 5 weeks of age and placed in the CLAMS. The ClAMS measured B Lococmotor activity, C energy expenditure and D respiratory quotient. E Brown adipose tissue uncoupling protein mRNA was measured at 16 weeks of age in male mice n = 6 fed either RC or HFD from 5 weeks of age. Data are represented mean ± SEM, (*P < 0.05).
Gastroenterology  , e1DOI: ( /j.gastro )
Copyright © 2010 AGA Institute Terms and Conditions