1. Rapid prenatal confirmation of LIT1 hypomethylation using a novel quantitative method (E-Q-PCR) in fetuses with Beckwith-Wiedemann syndrome impressed with ultrasonography 
Gwo-Chin Ma, Ph.D., Shuenn-Dyh Chang, M.D., Yu Chang, M.D., Shun-Ping Chang, M.S., Chin-Wen Yang, B.S., Ming-Jen Lee, M.D., Ph.D., Tsung-Hsien Lee, M.D., Ming Chen, M.D. 
Fertility and Sterility 
Volume 90, Issue 4, Pages (October 2008)
DOI: /j.fertnstert
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Schematic diagram of the E-Q-PCR assay for assessing the LIT1 methylation at chromosome 11p15.5. (A) Map of the differentially methylated regions (DMRs) at 11p15.5. Positions of the polymerase chain reaction (PCR)-amplified NotI-cut and NotI-uncut regions are marked in red and blue, respectively. (B) Representative experiments of the E-Q-PCR. Left panel: Sample from a healthy individual with a threshold cycle (Ct) difference of the NotI-cut (25.77) and NotI-uncut (24.87) regions of 0.9, methylation index (MI) was estimated at 53.59%. Right panel: Sample from a BWS fetus with LIT1 hypomethylation. His Ct difference of the NotI-cut (28.78) and NotI-uncut (25.32) regions is 3.46, MI is 9.09%. Notably, loss of DNA methylation at LIT1 results in the decrease of the starting DNA concentration of the NotI-cut region, increasing the Ct value. (C) The MI of LIT1 determined in samples from the BWS (Bf) and unaffected fetuses (Uf), from the mothers of the Bf (mBf) and Uf (mUf), and from other 20 unrelated healthy individuals (20U). The range of mean ± 2 SD for respective testing is delineated by the line. ∗∗∗P<.001.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions