1. Fig. 3. Thorase variants increase the frequency and amplitude of sEPSCs and mEPSCs.
Thorase variants increase the frequency and amplitude of sEPSCs and mEPSCs. (A) Representative traces of spontaneous glutamate-mediated excitatory postsynaptic currents (sEPSCs) recorded in heterozygous Thorase-deficient mice expressing WT Thorase (Het-WT; black) or the Thorase variants R9H, D221H, and E290K. (B to E) Mean cumulative probability distributions for sEPSC amplitude (B) and sEPSC frequency (D) recorded in prelimbic pyramidal neurons in brain slices from the prefrontal cortex of heterozygous Thorase-deficient mice expressing WT Thorase (Het-WT; black) or one of the three Thorase variants. (C and E) Mean sEPSC amplitude and sEPSC frequency, respectively. Means ± SEM (n ≥ 5). *P < 0.05; n.s., P > 0.10, ANOVA with Holm-Sidak post hoc test when compared to Het-WT mice (black). Power (1 − β error probability) = 0.8 to 1.0. (F) Representative traces of glutamate-mediated miniature excitatory postsynaptic currents (mEPSCs) recorded in WT mice (brown) or heterozygous Thorase-deficient mice expressing WT Thorase (Het-WT; black) or the Thorase variants R9H (orange), D221H (blue), and E290K (green). (G to J) Mean cumulative probability distributions for mEPSC amplitude (G) and mEPSC frequency (I) for prelimbic pyramidal neurons recorded in brain slices from the prefrontal cortex of WT mice (brown) or heterozygous Thorase-deficient mice expressing WT Thorase (Het-WT; black) or the Thorase variants R9H (orange), D221H (blue), and E290K (green). (H and J) Mean mEPSC amplitude and mEPSC frequency, respectively. Means ± SEM (n ≥ 4). *P < 0.05; n.s., P > 0.10, ANOVA with Holm-Sidak post hoc test when compared to Het-WT mice (black). Power (1 − β error probability) = 0.8.
George K. E. Umanah et al., Sci Transl Med 2017;9:eaah4985
Published by AAAS