1. Altered ultrastructure of mitochondrial membranes is strongly associated with unexplained asthenozoospermia 
Fiore Pelliccione, M.D., Alessandro Micillo, M.D., Giuliana Cordeschi, Ph.D., Anatolia D’Angeli, Ph.D., Stefano Necozione, M.D., Loredana Gandini, Ph.D., Andrea Lenzi, M.D., Felice Francavilla, M.D., Sandro Francavilla, M.D. 
Fertility and Sterility 
Volume 95, Issue 2, Pages (February 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Normal MP. (A) Longitudinal view with mitochondria regularly disposed in 13 concentric gyres. Scattered mitochondria show dilated spaces between cristae (arrows). (B) Elongated mitochondrion wrapped around the dense fibers of the axoneme, contains cristae closely apposed (arrows). Cristae run parallel to the outer membrane and are separated by a narrow intermembrane space (asterisks). Arrowhead points to an electron dense granule. (C) Cristae are arranged in a concentric system (arrows) in the longitudinal view of a normal MP.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Abnormal MP. (A) Mitochondria with dilated intermembrane space (asterisk). (B) All mitochondria, regularly arranged around the axoneme, show dilated intermembrane spaces (asterisks) between convoluted cristae. (C) Mitochondria irregularly arranged around the axoneme, show swollen intermembrane spaces (asterisks) and scattered irregularly arranged thin cristae (arrow). (D) Cross view and longitudinal view (E) of regularly arranged mitochondria showing a disaggregated inner structure involving all mitochondria in a single MP. (F) Agenesia of the MP in a case of dysplasia of the fibrous sheath. The fibrous sheath (FS) is distorted, and a short MP (asterisk) does not contain mitochondria that are irregularly amassed away from the tail structure (arrow).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Scattergrams showing the correlation of percentage of MPs showing mitochondria with normal membranes (% normal MP) and sperm FM. Filled circles indicate cases of systematic sperm tail defects. P <
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Box and whiskers plots of the percentage of MPs showing mitochondria with normal membranes (% normal MPs) in ejaculates with normal sperm forward motility (FM ≥50%; n = 15), in ejaculates with unexplained moderate asthenozoospermia (FM ≥10% and <50%; n = 27), and in ejaculates with severe asthenozoospermia (FM <10%) associated with systematic sperm tail defects (n = 14) or of unknown origin (n = 16). Values are medians (horizontal bars) with 25th and 75th percentiles (boxes); the “whiskers” indicate the nonoutlier range. Wilcoxon rank-sum test. P < , for the first and third boxes.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions