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Padova sequencing contribution:
100,000 Fosmid ends 4-5 runs 454-Titanium 2 slides SOLiD (10Kb inserts, 50x2 bases)
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Data production 2
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Mapping SOLiD reads on 454 contigs
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Sorting out the links
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Counting links to produce SOLID arches
4 possible ways to merge 2 fragments Count all possible merging betweenall the possible pairs of contigs
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Building a path to order the contigs
List of links (arches) 1: 9, 12 2: 6,18 3: 17, 20 4: 5 5: 4, 15 6: 2, 16 7: 14, 10 8: 11, 19 9: 1, 16 10: 7 11: 8, 15 12: 1, 17 13: 14, 20 14: 7, 13 15: 5, 11 16: 6, 9 17: 3, 12 18: 2, 19 19: 8, 18 20: 3, 13 From the above list it should be easily produced the following scaffold: – 20 – 13 – 14 –
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