1. The Thyroid Hormone Analogue KB2115 (Eprotirome) Prolongs Human Hair Growth (Anagen) Ex Vivo 
Attila Oláh, Jennifer Gherardini, Marta Bertolini, Jérémy Chéret, Leslie Ponce, Jennifer Kloepper, Tamás Bíró, Michael Soeberdt, Christoph Abels, Ralf Paus 
Journal of Investigative Dermatology 
Volume 136, Issue 8, Pages (August 2016)
DOI: /j.jid
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2. Figure 1
KB2115 promotes the anagen phase and increases proliferation of matrix keratinocytes in human hair follicles. (a) Results of microscopic hair cycle staging of three independent donors. Ratios of anagen and early, mid-, and late catagen hair follicles were calculated in each case. Data are shown as mean ± standard error of the mean of the three donors. (b) Percentages of proliferating (Ki67+) and apoptotic (TUNEL+) matrix keratinocytes were determined. Data are expressed as mean ± standard error of the mean of n = 36–37 HFs from three donors. ∗P < 0.05 and ∗∗∗P < versus control group. (c) Representative pictures of Ki67 (red) and TUNEL (green) double staining after the indicated treatments. Scale bar = 50 μm. Nuclei were counterstained by 4′-6-diamidino-2-phenylindole (DAPI; blue). HF, hair follicle; pM, pmol/L; n.s., not significant.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions

3. Figure 2
KB2115 tends to increase melanin synthesis, significantly increases tyrosinase activity, and decreases TGF-β2 immunoreactivity. (a, c, e) Signal intensities (determined as arbitrary units) of (a) Masson-Fontana histochemistry, (c) tyrosinase activity assay, and (e) TGF-β2 immunofluorescence were normalized to the control. Data are expressed as mean ± standard error of the mean of (a) n = 37–42, (c) n = 28–39, or (e) n = 29–36 hair follicles of three donors. ∗∗P < 0.01, ∗∗∗P < versus control group. (b, d, f) Representative images of (b) Masson-Fontana (black) histochemistry, (d) tyrosinase activity (red) assay, and (f) TGF-β2 (green) immunofluorescence. Nuclei were counterstained by (b) hematoxylin (blue) or by (d, f) 4′-6-diamidino-2-phenylindole (DAPI; blue). Scale bar = 50 μm. MF, Masson-Fontana; n.s., not significant; pM, pmol/L; TGF, transforming growth factor; Tyr, tyrosinase.
Journal of Investigative Dermatology  , DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions