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Biosafety training BSL2/BSL3 TB

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Presentation on theme: "Biosafety training BSL2/BSL3 TB"— Presentation transcript:

1 Biosafety training BSL2/BSL3 TB
10/11/2011

2 Policies needed for Biosafety in the Laboratory
TB Lines of Defense in the Laboratory 2

3 Biosafety depends on Good Laboratory Practices
NOTHING can totally eliminate the safety risk associated with the TB laboratory Good laboratory practices with emphasis on biosafety significantly reduces the risk of laboratory-acquired infection Specialized equipment aids good laboratory practice but does NOT replace it 3

4 Types of cabinets: General
Product Protection Personal protection Product and personal protection

5 Types of cabinets: Biological safety cabinets
Class II A HEPA-Filter Pre-filter Class II A (Double exhaust) Class II B

6 Types of cabinets: Biological safety cabinets
Flow pattern 30% Downflow Exhaust Inflow Recirculation Pressure plenum 70%

7 Working safely in your BSC
Escoglobal video Air flow patterns in a BSC GenomicaUASLP

8 The primary risks to TB Laboratorians are :
AEROSOLS Aerosols may include droplet nuclei containing 1-3 live bacilli that remain suspended in the air – a real danger for laboratory staff 8

9 Manipulations that produce Aerosols
Pipetting Mixing Shaking Centrifugation Grinding Vortexing Pouring Use of squeeze bottles during processing Flaming loops or wet slides Opening tubes at non-ambient temperatures or pressures Pipet tip 9

10 How to Minimize Aerosols
Always open and manipulate specimens and cultures in a certified biosafety cabinet (BSC) Use disposable loops or incinerators to sterilize loops Work over absorbent material soaked in disinfectant Use centrifuge safety cups and open them in the BSC Never express all the liquid in the pipette (leave the last bit inside the tip) Use caution when disrupting bubbles in tubes of liquid media 10

11 Specimen processing Can you identify any breaks in protocol?

12 Specimen processing Can you identify any breaks in protocol?

13 Specimen processing Can you identify any breaks in protocol?

14 Specimen processing Can you identify any breaks in protocol?

15 Specimen processing Can you identify any breaks in protocol?

16 Specimen processing Can you identify any breaks in protocol?

17 Specimen processing

18 Specimen processing

19 Specimen processing


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