1. Biosafety training BSL2/BSL3 TB
10/11/2011

2. Policies needed for Biosafety in the LaboratoryTB
Lines of Defense in the Laboratory 2

3. Biosafety depends on Good Laboratory Practices
NOTHING can totally eliminate the safety risk associated with the TB laboratory
Good laboratory practices with emphasis on biosafety significantly reduces the risk of laboratory-acquired infection
Specialized equipment aids good laboratory practice but does NOT replace it 3

4. Types of cabinets: General
Product Protection
Personal protection
Product and personal protection

5. Types of cabinets: Biological safety cabinets
Class II A
HEPA-Filter
Pre-filter
Class II A (Double exhaust)
Class II B

6. Types of cabinets: Biological safety cabinets
Flow pattern
30%
Downflow
Exhaust
Inflow
Recirculation
Pressure plenum
70%

7. Working safely in your BSC
Escoglobal video
Air flow patterns in a BSC
GenomicaUASLP

8. The primary risks to TB Laboratorians are :
AEROSOLS
Aerosols may include droplet nuclei containing 1-3 live bacilli that remain suspended in the air – a real danger for laboratory staff 8

9. Manipulations that produce Aerosols
Pipetting
Mixing
Shaking
Centrifugation
Grinding
Vortexing
Pouring
Use of squeeze bottles during processing
Flaming loops or wet slides
Opening tubes at non-ambient temperatures or pressures
Pipet tip 9

10. How to Minimize Aerosols
Always open and manipulate specimens and cultures in a certified biosafety cabinet (BSC)
Use disposable loops or incinerators to sterilize loops
Work over absorbent material soaked in disinfectant
Use centrifuge safety cups and open them in the BSC
Never express all the liquid in the pipette (leave the last bit inside the tip)
Use caution when disrupting bubbles in tubes of liquid media 10

11. Specimen processing
Can you identify any breaks in protocol?

12. Specimen processing
Can you identify any breaks in protocol?

13. Specimen processing
Can you identify any breaks in protocol?

14. Specimen processing
Can you identify any breaks in protocol?

15. Specimen processing
Can you identify any breaks in protocol?

16. Specimen processing
Can you identify any breaks in protocol?

17. Specimen processing

18. Specimen processing

19. Specimen processing