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Published byFlorian Weber Modified over 6 years ago
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A B Figure S1 DU145 PC-3 22Rv1 RWPE-1 BPH1 Relative levels
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A B Figure S2 RWPE-1 RWPE-1 % Methylated Species % Methylated Species
DU145 DU145 % Methylated Species % Methylated Species
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A B Figure S3 RWPE-1 RWPE-1 % Methylated Species % Methylated Species
BPH1 BPH1 % Methylated Species % Methylated Species
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A B Figure S4 * * * DU145 PC-3 DU145 PC-3 % Annexin V positive cells
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A B C D E Figure S5 ** ** ** ** ** Colonies/well Cells/field
Relative growth Time (hours) D E ** Cells/field Scramble siMMSET pool siMMSET c-ter
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Relative E-cadherin protein
Figure S6 A Scramble siMMSET pool siMMSET c-ter B C Vimentin GAPDH Scramble MMSET pool siRNA: MMSET c-ter Occludin E-cadherin Relative expression * ** ** * ** ** Vimentin Fibronectin 1 N-cadherin * * ** Relative E-cadherin protein Relative expression γ-catenin E-cadherin Occludin
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A B Figure S7 RWPE-1 *** Relative expression * ** DU145
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A B C Figure S8 Relative TWIST1 expression t(4;14)+ t(4;14)- p =0.005
400 350 300 250 Relative TWIST1 expression Relative TWIST1 expression 200 150 100 50
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A B C D Figure S9 siTwist1 #1 siTwist1 #2 Scramble ** ** Vimentin
Cells/field ** Cells/field Occludin Vimentin E-cadherin GAPDH siRNA: Scr Twist#2 Twist#1
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A B Figure S10 MMSET H3K36me2 ** % Enrichment ** % Enrichment MMSET
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