1. A B Figure S1 DU145 PC-3 22Rv1 RWPE-1 BPH1 Relative levels

2. A B Figure S2 RWPE-1 RWPE-1 % Methylated Species % Methylated Species
DU145
DU145
% Methylated Species
% Methylated Species

3. A B Figure S3 RWPE-1 RWPE-1 % Methylated Species % Methylated Species
BPH1
BPH1
% Methylated Species
% Methylated Species

4. A B Figure S4 * * * DU145 PC-3 DU145 PC-3 % Annexin V positive cells

5. A B C D E Figure S5 ** ** ** ** ** Colonies/well Cells/field
Relative growth
Time (hours) D E **
Cells/field
Scramble
siMMSET pool
siMMSET c-ter

6. Relative E-cadherin protein
Figure S6 A
Scramble
siMMSET pool
siMMSET c-ter B C
Vimentin
GAPDH
Scramble
MMSET pool
siRNA:
MMSET c-ter
Occludin
E-cadherin
Relative expression * ** ** * ** **
Vimentin
Fibronectin 1
N-cadherin * * **
Relative E-cadherin protein
Relative expression
γ-catenin
E-cadherin
Occludin

7. A B Figure S7 RWPE-1 *** Relative expression * ** DU145

8. A B C Figure S8 Relative TWIST1 expression t(4;14)+ t(4;14)- p =0.005
400
350
300
250
Relative TWIST1 expression
Relative TWIST1 expression
200
150
100 50

9. A B C D Figure S9 siTwist1 #1 siTwist1 #2 Scramble ** ** Vimentin
Cells/field **
Cells/field
Occludin
Vimentin
E-cadherin
GAPDH
siRNA:
Scr
Twist#2
Twist#1

10. A B Figure S10 MMSET H3K36me2 ** % Enrichment ** % Enrichment MMSET