1. Volume 129, Issue 4, Pages 1274-1281 (October 2005)
A Comparative Analysis by SAGE of Gene Expression Profiles of Barrett’s Esophagus, Normal Squamous Esophagus, and Gastric Cardia 
Jantine W.P.M. van Baal, Francesca Milano, Agnieszka M. Rygiel, Jacques J.G.H.M. Bergman, Wilda D. Rosmolen, Sander J.H. van Deventer, Kenneth K. Wang, Maikel P. Peppelenbosch, Kausilia K. Krishnadath 
Gastroenterology 
Volume 129, Issue 4, Pages (October 2005)
DOI: /j.gastro
Copyright © 2005 American Gastroenterological Association Terms and Conditions

2. Figure 1
(A) RT-PCR on RNA from BE (BA) and squamous esophagus (SQ) biopsy specimens from different patients shows that FABP1, annexin A10, TFF1, TFF2, TFF3, and galectin-4 are highly expressed in the biopsy specimens of BE but virtually absent in squamous epithelium. β-actin and β2-microglobulin were used as a control. (B) Quantification of RT-PCR results shows that FABP1, annexin A10, TFF1, TFF2, TFF3, and galectin-4 in BE and squamous esophagus of 20 patients are significantly higher expressed in the biopsy specimens of BE (2-tailed paired t tests; *P < .05, **P < .01). The gene expression levels were determined by the ratio of signal intensity of the mRNA to that of the β-actin. Data are expressed as means ± SEM.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2005 American Gastroenterological Association Terms and Conditions

3. Figure 2
Keratin expression patterns in the SAGE libraries of BE, normal squamous esophagus, and gastric cardia. CKs 4, 6A, 6B, and 13 were expressed higher in squamous esophagus. CKs 7, 8, 18, 19, and 20 were expressed higher in BE compared with squamous esophagus. CK18 and CK20 were highly expressed in both the BE and the gastric cardia SAGE libraries.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2005 American Gastroenterological Association Terms and Conditions

4. Figure 3
(A) Validation by RT-PCR on RNA from BE (BA) and squamous esophagus (SQ) biopsy specimens from several patients. RT-PCR shows a higher expression of CK7 and CK20 in BE versus squamous esophagus. (B) Quantification of RT-PCR results of gene expression of CK7 and CK20 in BE and squamous esophagus of 20 patients shows that these are significantly higher expressed in the BE biopsy specimens (2-tailed paired t tests; *P < .05, **P < .01). The gene expression levels were determined by the ratio of signal intensity of the mRNA to that of the β-actin. Data are expressed as means ± SEM.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2005 American Gastroenterological Association Terms and Conditions

5. Figure 4
Immunoblot analysis of CK 5/6, 7, 8, 10/13, 18, and 20 expression in squamous esophagus, BE, and gastric cardia shows higher expression levels of CKs 5/6 and 10/13 in squamous epithelium, while CKs 7, 8, and 20 are highly expressed in BE and less in the cardia biopsy specimens. β-actin was used as a control.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2005 American Gastroenterological Association Terms and Conditions