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Molecular Typing of West Nile Virus, Dengue, and St

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Presentation on theme: "Molecular Typing of West Nile Virus, Dengue, and St"— Presentation transcript:

1 Molecular Typing of West Nile Virus, Dengue, and St
Molecular Typing of West Nile Virus, Dengue, and St. Louis Encephalitis Using Multiplex Sequencing  Thuraiayah Vinayagamoorthy, Kirk Mulatz, Michael Drebot, Roger Hodkinson  The Journal of Molecular Diagnostics  Volume 7, Issue 2, Pages (May 2005) DOI: /S (10) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Stages of the MultiGEN process and the basic scientific principles. These include: Multiplex amplification, Multiplex sequencing, electrophoresis and computer analyzed electropherogram that is ready for BLAST search. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Nucleotide sequences from two selected regions from the 266-bp amplicon. A: Signature nucleotide sequences that identify WNV and St. Louis encephalitis. B: Signature nucleotide sequences that identify Dengue types 1–4. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Electropherograms showing nucleotide sequences from six RNA extracts analyzed. A: WNV; B-E: Dengue serotypes; F: St. Louis encephalitis. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

5 Figure 4 A: Region of the WNV genome from which two amplicons were generated, positioning of the sequencing primers, and the electropherograms produced from two samples. B: Photograph of an ethidium bromide stained 2% agarose gel showing the DNA bands of the two amplicons. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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