1. Detection of BRAF p.V600E Mutations in Melanomas
Emeline Colomba, Zofia Hélias-Rodzewicz, Andreas Von Deimling, Cristi Marin, Nathalie Terrones, Dominique Pechaud, Sylvie Surel, Jean-François Côté, Frédérique Peschaud, David Capper, Hélène Blons, Ute Zimmermann, Thierry Clerici, Philippe Saiag, Jean- François Emile 
The Journal of Molecular Diagnostics 
Volume 15, Issue 1, Pages (January 2013)
DOI: /j.jmoldx
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Determination of the reference responses by using the results of the four methods.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
False-negative results with Sanger sequencing performed on samples containing >60% of tumor cells. Left column: Five chromatograms with a negative control (Wild type), three false-negative chromatograms (Y , Y , and Y ), and a positive control (p.V600E). Right column: Pyrosequencing (top) and real-time PCR (bottom) of the same cases. The mutant allele (red curve) was only detectable with PNA inhibition of the WT allele. The injection sequence of programs was 5′-GACAACGATGTCAATCTC-3′.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4. Figure 3
Detection of p.V600K with p.V600E-specific real-time PCR. Amplification curves of four cases with a negative control, two p.V600K cases, and a p.V600E-positive control. Without PNAs, amplification of both mutated (red) and WT (green) alleles was obvious on the p.V600E-positive control, whereas only the WT were detectable on the three other samples. The p.V600K mutations (red curves) were only detectable with the PNA inhibition of WT allele amplification.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

5. Figure 4
VE1 IHC of melanoma tissue microarray samples. Top to bottom: Two BRAF p.V600E-mutated positive melanomas with a strong cytoplasmic red staining of melanoma cells. Two BRAF WT cases negative for VE1, the first one containing single positively stained macrophages, an artifact easily distinguishable from the positive tumor staining in p.V600E mutated melanomas, and the second one with a high amount of melanin. The bottom case is one of the seven cases that were considered as not interpretable by IHC because of an ambiguous, mostly nuclear staining reaction.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions