1. Holliday model Strand exchange and Heteroduplex DNA formation
HJ resolution
Mismatch repair

2. Meselson-Radding
1975

3. Szostak
Orr-Weaver
Rothstein
Stahl (1983) model

4. Synthesis Dependent Strand Annealing

5. Rad51-dependent ectopic (interchromosomal) gene conversion in haploid cells
HO endonuclease-induced DSB
Non-crossover
Crossover

6. Crossovers and Noncrossovers occur with different kinetics
wild type
noncrossover
crossover
crossover
noncrossover
Crossovers and Noncrossovers occur with different kinetics
Analogous results in yeast meiosis (Allers and Lichten 2001)

7. Modified SDSA
SzOrRoST (1983)

8. Meiotic recombination
Axial element
Spo11
Mre11
Rad50
etc
DSB
Synaptonemal
complex
Timing of meiotic DSBs: 2 hr after replication.
Suggests some structures are assembled during replication that are needed to create DSB

9. Kinetics of meiotic recombination initiated by a DSB
Double HJ
2D gels
2D gels, as used to examine replication

10. Analysis of Joint Molecule intermediates by denaturing gel electrophoresis
Is it this ?
Or this?
This