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ADVANCED TARGETING SYSTEMS for specific elimination of cell types
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1. Get an antibody specific to your cell type Or, you can use a
growth factor, peptide, or cytokine ANTIBODY 7
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2. Use a potent toxin that is safe in the lab, but lethal
inside a cell. SAPORIN 7
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3. ATS puts them together to make a powerful targeting agent 7
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Targeted Toxins can be used in vitro or in vivo 7
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Targeted Toxins can be used in vitro or in vivo 7
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The antibody seeks out its target receptor on the cell’s surface. 7
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The antibody seeks out its target receptor on the cell’s surface. 7
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Without its escort, SAPORIN cannot get inside a cell. 7
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Without its escort, SAPORIN cannot get inside a cell. 7
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Once attached to its target receptor, the conjugate is internalized. 7
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Once attached to its target receptor, the conjugate is internalized. 7
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Once attached to its target receptor, the conjugate is internalized. 7
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Once attached to its target receptor, the conjugate is internalized. 7
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have the target receptor will not be affected.
Cells which do not have the target receptor will not be affected. 7
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have the target receptor will not be affected.
Cells which do not have the target receptor will not be affected. 7
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have the target receptor will not be affected.
Cells which do not have the target receptor will not be affected. 7
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have the target receptor will not be affected.
Cells which do not have the target receptor will not be affected. 7
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have the target receptor will not be affected.
Cells which do not have the target receptor will not be affected. 7
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The SAPORIN conjugate enters the cell.
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The SAPORIN conjugate enters the cell.
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The SAPORIN conjugate enters the cell.
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The SAPORIN conjugate enters the cell.
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Once inside, SAPORIN will inactivate the ribosome.
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Once inside, SAPORIN will inactivate the ribosome.
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Once inside, SAPORIN will inactivate the ribosome.
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The result is cell death.
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The result is cell death.
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The result is cell death.
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The conjugate is carried inside the cell.
coated vesicle 7
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coated vesicle 7
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coated vesicle 7
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coated vesicle 7
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coated vesicle 7
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Saporin escapes Ribosomes Golgi 7
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Ribosomes Golgi 7
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Ribosomes Golgi 7
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SAPORIN inactivates the ribosome
Ribosomes SAPORIN inactivates the ribosome Golgi 7
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RIP Ribosomes SAPORIN inactivates the ribosome R I P Golgi 7
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SAPORIN inactivates the ribosome
R I P 7
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SAPORIN inactivates the ribosome
R I P R I P 7
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R I P R I P 7
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R I P R I P R I P 7
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The result is Cell Death R I P R I P R I P 7
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R I P R I P R I P R I P 7
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R I P R I P R I P R I P 7
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R I P R I P R I P R I P R I P 7
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R I P R I P R I P R I P R I P 7
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R I P R I P R I P R I P R I P R I P 7
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R I P R I P R I P R I P R I P R I P 7
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R I P R I P R I P R I P R I P R I P R I P 7
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R I P R I P R I P R I P R I P R I P R I P 7
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ADVANCED TARGETING SYSTEMS for specific elimination of cell types
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Saporin Saporin (pronounced sap – r – n) e e
⏝ Saporin (pronounced sap – r – n) e e Saporin is a ribosome-inactivating protein. Molecular weight – 30 kDa From the seeds of the plant, Saponaria officinalis Saporin is safe for laboratory use under normal safety conditions LD50 in mice – 4 mg/kg Saporin does not have a method of cell entry on its own, except through bulk-phase endocytosis
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Advanced Targeting Systems
also provides Custom Conjugations to use your Targeting Agent to deliver any Payload. Targeting Agent * Antibody Peptide Ligand Aptamer Growth Factor Cytokine Payload Toxin Drug Killing Agent Activating Agent Reversible Agent * Anything that is recognized on the cell surface and internalizes
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