1. Human sPD1-based HIV-1 Gag-fusion DNA vaccine induces high frequency of broadly reactive T cell responses in mice and rhesus macaques
Samantha MY Chen
The mosaic-like immunogen design was based on the circulating HIV-1 subtypes in China
The novel DNA vaccine contain human sPD1 fused together with two Gag p41
The vaccine-induced immunogenic response and protection was explored in BALB/c mice
We further investigated this DNA vaccine in rhesus macaques to prove concept.
JCI. 2013;123(6):
HKU5/CRF/13G and ITS/194/13

2. huPD1-Gag fusion DNA vaccine induces strong T-cell response in BALB/c
Partial protection against EcoHIV
HKU5/CRF/13G and ITS/194/13

3. Broad T-cell response in Rhesus macaques after vaccination
Red and orange/yellow are epitope specific CD8 T cell epitopes associated with HIV control.
Green/light blue, purple and blue targets the initial immunodominant CD8 T cell response in early HIV-1 infection.
Pink/grey is the immunoprevalent HIV CD4 T cell epitope observed in elite controllers
T-cell epitope mapping
Boosting effect of DNA vaccine
Broad reactivity to HIV subtypes
HKU5/CRF/13G and ITS/194/13