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G&H : Oct. 2001.

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Presentation on theme: "G&H : Oct. 2001."— Presentation transcript:

1 G&H : Oct. 2001

2 G&H : In vitro experiment 2001
1 variety : PRI* 3 mediums (ref. 611) (S30-S60-S120) 3 induction conditions (cold period) 3 environmental factors (F, F+i, 27°C) 24 treatments (3 months cold is late) * MES: 6 treatments only, 0 and 3 months cold induction, on and 120 g/l sucrose

3 In vitro cultures (PRI)
G&H : In vitro experiment 2001 In vitro cultures (PRI) Sucrose gradient ( g/l)

4 G&H : In vitro experiment 2001
Analysis of Variance Source Sum-of-Squares df Mean-Square F-ratio P COLD SUCROSE Error Least Squares Means F F+i F27 CLIMATE 1 2 ALLIIN 30 60 120 SUCROSE 1 2

5 G&H : In vitro experiment 2001
Alliin content of PRI basal parts of garlic plants grown in vitro under various climatic conditions and on distinct sucrose concentrations (g/l) in the culture medium

6 Mineral base of MS, replacing SO4 ions by Cl2
G&H : In vitro experiments 2002 Mineral base of MS, replacing SO4 ions by Cl2 EXP. in vitro II , Medium ref. 633, Sucrose 6%, Agar 0,65% EXP. in vitro III , Medium ref. 648, Sucrose 6%, liquid medium 0-3-6 or meq SO4 MES PRI MOR

7 Greenhouse conditions 2001
PRI MOR

8 G&H : Greenhouse conditions 2001

9 G&H : Field trial FR2001 Least Squares Means 1 2 3 4 S treatment 71 79
87 95 103 Bulb weight (g) Analysis of Variance Sum-of-Squares df Mean-Square F-ratio P S LEVEL Error

10 G&H : Field trial FR2001 Soil analyses performed by INRA Laboratories at ARRAS 20/06/01 28/08/01 21/02/01 25/08/00 14/05/01 Sulphur (France) 100 200 400 Treatment Total 217 149 189 156 172 187 168 127 188 114 Water soluble 9 7 5,6 8 9,7 11 12,2 16 20,8 22


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