Presentation is loading. Please wait.

Presentation is loading. Please wait.

Arabinogalactan isolated from cowshed dust extract protects mice from allergic airway inflammation and sensitization  Marcus Peters, PhD, Marion Kauth,

Published byAurora Haapasalo Modified over 6 years ago

Similar presentations

Presentation on theme: "Arabinogalactan isolated from cowshed dust extract protects mice from allergic airway inflammation and sensitization  Marcus Peters, PhD, Marion Kauth,"— Presentation transcript:

1 Arabinogalactan isolated from cowshed dust extract protects mice from allergic airway inflammation and sensitization  Marcus Peters, PhD, Marion Kauth, PhD, Olaf Scherner, PhD, Kirsten Gehlhar, PhD, Imke Steffen, MSc, Pia Wentker, MSc, Erika von Mutius, MD, Otto Holst, PhD, Albrecht Bufe, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 126, Issue 3, Pages e4 (September 2010) DOI: /j.jaci Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Mice were treated during sensitization and airway challenge with 50 μg CDE, high-molecular-mass fraction (P1), 1 μg arabinogalactan (AG) isolated from P1, or supernatant of the Yariv precipitation. Subsequently, mice were evaluated for eosinophilic airway inflammation (A), cytokine production (B), and IgE levels (C). Results are presented as means with SDs (n = 4). ∗P < .05. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Mice were treated during sensitization and airway challenge with either extract from A pratensis or arabinogalactan (AG) isolated from this extract. Subsequently, mice were evaluated for eosinophilic airway inflammation (A), cytokine production (B), and IgE production in BAL fluid (C). Results are presented as means with SDs (n = 4). ∗P < .05. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Mice were either sham-treated (n = 10) or treated by intranasal application of 5 μg arabinogalactan (AG; n = 10). Airway hyperreactivity was measured invasively. Means ± SEMs are shown ∗P < .05; ∗∗P < .01; ∗∗∗P < .001 (A). Subsequently, lung sections were stained by Alcian blue/periodic acid-Schiff reagent (B). Goblet cells were counted in 4 different lung slices from each mouse and given as cells per millimeter basement membrane. Means ± SDs are shown (n = 5). Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Mice were treated by inhalation of arabinogalactan (AG) isolated from gum arabic or larch during sensitization and airway challenge with OVA. Subsequently, mice were evaluated for eosinophilic airway inflammation (A), IL-5 production (B), and IgE production in BAL fluid (C). Results are presented as means with SDs (n = 4). Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Analysis of activity of arabinogalactan (AG) from A pratensis on mouse BMDCs. A, IL-10 production of BMDCs in vitro after stimulation with 10 μg/mL AG. B and C, AG-treated or untreated BMDCs were used to sensitize mice via the airways. After challenge of mice, they were evaluated for eosinophilic airway inflammation (B) and cytokine production of splenic lymphocytes (C). Median is shown. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig 6 One dimensional hydrogen-1 NMR spectrum of AG from A pratensis (27°C, deuterated water) (A) and of arabinogalactan (AG) from Larix (B). The anomeric protons of α- L-Araf, β-L-Araf, and β-D-Galp residues are labeled. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig 7 Analysis of activity of arabinogalactan (AG) treated with oxalic acid at 60°C, 18 hours, or control treated by omitting oxalic acid. Mice were treated by inhalation of AG during sensitization and airway challenge. Subsequently, mice were evaluated for eosinophilic airway inflammation (A), IL-5 production (B), and OVA-specific IgE in BAL fluid (C). Means with SDs are shown (n = 5). ∗P < .05; ∗∗P < .01. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Depiction of treatment protocol. OVA i. p
Depiction of treatment protocol. OVA i.p., Systemic immunization with OVA adsorbed in aluminum hydroxide. OVA-aerosol, Challenges with 1% OVA aerosol via the airways. AR, Measurements of airway responsiveness. Analysis, Harvest of BAL cells, serum, and splenocytes for analysis. i.n., Intranasal. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

10 LPS was applied to mice intranasally during sensitization and airway challenge with ovalbumin. Subsequently mice were evaluated for eosinophilic airway inflammation (A), IL-5 production (B), and IgE production in BAL fluid (C). Results are presented as means with SDs calculated from 4 mice per group. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

11 Neutralization of produced IL-10 partially restores sensitizing capacity of arabinogalactan (AG)–treated BMDCs. BMDCs were treated with AG, and released IL-10 was blocked by a IL-10–neutralizing antibody (OVA + AG + anti–IL-10). Control cells were treated with AG in the presence of an isotype control antibody. Subsequently, cells were used to sensitize mice via the airways. Three days after challenge with OVA aerosol, all mice were evaluated for eosinophilic airway inflammation. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Arabinogalactan isolated from cowshed dust extract protects mice from allergic airway inflammation and sensitization  Marcus Peters, PhD, Marion Kauth,"

Similar presentations

Dual effects of vitamin D–induced alteration of TH1/TH2 cytokine expression  Victor Matheu, MD, Ove Bäck, MD, PhD, Emma Mondoc, BSc, Shohreh Issazadeh-Navikas,

Dual effects of vitamin D–induced alteration of TH1/TH2 cytokine expression  Victor Matheu, MD, Ove Bäck, MD, PhD, Emma Mondoc, BSc, Shohreh Issazadeh-Navikas,
Poly (ADP-ribose) polymerase 14 and its enzyme activity regulates TH2 differentiation and allergic airway disease  Purvi Mehrotra, PhD, Andrew Hollenbeck,

Poly (ADP-ribose) polymerase 14 and its enzyme activity regulates TH2 differentiation and allergic airway disease  Purvi Mehrotra, PhD, Andrew Hollenbeck,
Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitis  Pei-Chi Chen, MSc,

Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitis  Pei-Chi Chen, MSc,
Maternal immune response to helminth infection during pregnancy determines offspring susceptibility to allergic airway inflammation  Kathrin Straubinger,

Maternal immune response to helminth infection during pregnancy determines offspring susceptibility to allergic airway inflammation  Kathrin Straubinger,
IL-4 induces IL-13–independent allergic airway inflammation

IL-4 induces IL-13–independent allergic airway inflammation
Effective prevention and therapy of experimental allergic asthma using a GATA-3– specific DNAzyme  Serdar Sel, MD, Michael Wegmann, PhD, Tanja Dicke, MSc,

Effective prevention and therapy of experimental allergic asthma using a GATA-3– specific DNAzyme  Serdar Sel, MD, Michael Wegmann, PhD, Tanja Dicke, MSc,
The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.

The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.
Regulatory B cells prevent and reverse allergic airway inflammation via FoxP3-positive T regulatory cells in a murine model  Sylvie Amu, PhD, Sean P.

Regulatory B cells prevent and reverse allergic airway inflammation via FoxP3-positive T regulatory cells in a murine model  Sylvie Amu, PhD, Sean P.
The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.

The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.
Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.

Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.
Interferon response factor 3 is essential for house dust mite–induced airway allergy  Thomas Marichal, DVM, Denis Bedoret, DVM, PhD, Claire Mesnil, DVM,

Interferon response factor 3 is essential for house dust mite–induced airway allergy  Thomas Marichal, DVM, Denis Bedoret, DVM, PhD, Claire Mesnil, DVM,
Selective control of SIRP-α–positive airway dendritic cell trafficking through CD47 is critical for the development of TH2-mediated allergic inflammation 

Selective control of SIRP-α–positive airway dendritic cell trafficking through CD47 is critical for the development of TH2-mediated allergic inflammation 
Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation  Yuki Nakamura,

Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation  Yuki Nakamura,
Innate IL-13–producing nuocytes arise during allergic lung inflammation and contribute to airways hyperreactivity  Jillian L. Barlow, PhD, Agustin Bellosi,

Innate IL-13–producing nuocytes arise during allergic lung inflammation and contribute to airways hyperreactivity  Jillian L. Barlow, PhD, Agustin Bellosi,
Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C.

Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C.
Frank Kirstein, PhD, Natalie E

Frank Kirstein, PhD, Natalie E
Protective role of nuclear factor of activated T cells 2 in CD8+ long-lived memory T cells in an allergy model  Roman Karwot, PhD, Joachim H. Maxeiner,

Protective role of nuclear factor of activated T cells 2 in CD8+ long-lived memory T cells in an allergy model  Roman Karwot, PhD, Joachim H. Maxeiner,
Dexamethasone induces ω3-derived immunoresolvents driving resolution of allergic airway inflammation  Katerina Pyrillou, PhD, Aikaterini-Dimitra Chairakaki,

Dexamethasone induces ω3-derived immunoresolvents driving resolution of allergic airway inflammation  Katerina Pyrillou, PhD, Aikaterini-Dimitra Chairakaki,
Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro  Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.
Compartmentalized chemokine-dependent regulatory T-cell inhibition of allergic pulmonary inflammation  Roshi Afshar, PhD, James P. Strassner, BS, Edward.

Compartmentalized chemokine-dependent regulatory T-cell inhibition of allergic pulmonary inflammation  Roshi Afshar, PhD, James P. Strassner, BS, Edward.

Similar presentations

Ads by Google