1. a b
MCF-7 TR2
MCF-7 TR2
(Fold change)
MTT Assay ,
(Fold change)
MTT Assay
, ,
GW 4064 µM
CDCA µM c
HER2
β-Actin
CDCA µM
Supplemental Figure 2: MTT growth assays in MCF-7 TR2 cells treated with vehicle (-) or increasing doses of CDCA (12, µM) (a) or GW4064 (0,3-3-6,5-10 µM) (b) for 7 days. Cell proliferation is expressed as fold change ± SD relative to vehicle treated cells, and is representative of three different experiments each performed in triplicate. (c) MCF-7 TR2 cells were treated for 24h with vehicle (-) or CDCA 25 and 50µM before lysis. Equal amounts of total cellular extract were analyzed for HER2 levels by Western blotting. β-Actin was used as loading control.