1. Protein Structure

2. Biology/Chemistry of Protein Structure
Primary
Secondary
Tertiary
Quaternary
Assembly
Folding
Packing
Interaction
S T R U C T U R E
P R O C E S S

3. Primary Structure - Sequence

4. FOLDING

5. Basic Secondary structure
α-helix
β-sheet
Secondary structures, α-helix and β-sheet, have regular hydrogen-bonding patterns.

6. 1’ > 2’ > 3’ > 4’ > …
Tertiary structure
Quaternary structure

7. X - Ray
X-ray Diffraction Pattern for a Protein

8. X-Ray

9. NMR – there is no right answer

10. THE database of experimentally-derived structures of proteins, nucleic acids, and complexes
Access to structural information
Visualization
Analysis

11. PDB Growth and Content Experimental Methods: Macromolecules:
X-Ray Crystallography
NMR
more…
Macromolecules:
Proteins
Nucleic acids
Carbohydrates
Protein-nucleic acid complexes
Protein-carbohydrate complexes

12. PDB Homepage

13. An Example – 2ARA

14. Search Methods

15. Advanced Search

16. Sequence Search

17. Example Results