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Daniel D. Myers, DVM, MPH, Angela E. Hawley, MS, Diana M

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Presentation on theme: "Daniel D. Myers, DVM, MPH, Angela E. Hawley, MS, Diana M"— Presentation transcript:

1 P-selectin and leukocyte microparticles are associated with venous thrombogenesis 
Daniel D. Myers, DVM, MPH, Angela E. Hawley, MS, Diana M. Farris, LVT, Shirley K. Wrobleski, BS, Porama Thanaporn, BSE, Robert G. Schaub, PhD, Denisa D. Wagner, PhD, Anjali Kumar, PhD, Thomas W. Wakefield, MD  Journal of Vascular Surgery  Volume 38, Issue 5, Pages (November 2003) DOI: /S (03)

2 Fig 1 Experimental protocol. WT, Wild type; ^CT, mice with high circulating levels of soluble P-selectin; PKO, P-selectin gene-deleted mice; EPKO, double gene-deleted mice. Journal of Vascular Surgery  , DOI: ( /S (03) )

3 Fig 2 Thrombus mass at day 2 and day 6 in shams, wild type mice (WT), mice with high circulating levels of soluble P-selectin (^CT) mice, P-selectin gene-deleted mice (PKO), and double gene-deleted mice (EPKO). Shams included WT, ^CT, and PKO groups. n, Number of animals per group. Journal of Vascular Surgery  , DOI: ( /S (03) )

4 Fig 3 Morphometric evaluation of inflammatory cells in the vein wall at day 2 and day 6 in shams, wild type (WT) mice, mice with high circulating levels of soluble P-selectin (^CT) mice, P-selectin gene-deleted mice (PKO), and double gene-deleted mice (EPKO). Shams included WT, ^CT, and PKO groups. n, Number of animals per group; neut, neutrophils; mono, monocytes; TC, total inflammatory cells. Journal of Vascular Surgery  , DOI: ( /S (03) )

5 Fig 4 Fluorescence-activated cell scanning analysis with negative controls located on the left, and study specimens on the right. Shown is a representative example of each group at day 2 (A) and day 6 (B) in wild type (WT) mice, mice with high circulating levels of soluble P-selectin (^CT) mice, P-selectin gene-deleted mice (PKO), and double gene-deleted mice (EPKO). Journal of Vascular Surgery  , DOI: ( /S (03) )

6 Fig 4 Fluorescence-activated cell scanning analysis with negative controls located on the left, and study specimens on the right. Shown is a representative example of each group at day 2 (A) and day 6 (B) in wild type (WT) mice, mice with high circulating levels of soluble P-selectin (^CT) mice, P-selectin gene-deleted mice (PKO), and double gene-deleted mice (EPKO). Journal of Vascular Surgery  , DOI: ( /S (03) )

7 Fig 5 Soluble P-selectin levels at day 2 and day 6 in sham mice, wild type (WT) mice, mice with high circulating levels of soluble P-selectin (^CT) mice, P-selectin gene-deleted mice (PKO), and double gene-deleted mice (EPKO). Shams at day 2 include WT (6.01 ng/mg), ^CT (27.6 ng/mg), and PKO (0 ng/mg); shams at day 6 include WT (7.8 ng/mg), ^CT (27.2 ng/mg), and PKO (0 ng/mg). n, Number of animals per group. Journal of Vascular Surgery  , DOI: ( /S (03) )

8 Fig 6 Soluble E-selectin levels at day 2 and day 6 in sham mice, wild type (WT) mice, mice with high circulating levels of soluble P-selectin (^CT) mice, P-selectin gene-deleted mice (PKO), and double gene-deleted mice (EPKO). Shams at day 2 include WT (1284 pg/mg), ^CT (1023 pg/mg), and PKO (567 pg/mg); shams at day 6 include WT (1688 pg/mg), ^CT (1189 pg/mg), and PKO (615 pg/mg). n, Number of animals per group. Journal of Vascular Surgery  , DOI: ( /S (03) )

9 Fig 7 Thrombus mass analysis at day 2 and day 6 in wild type mice (WT), mice with high circulating levels of soluble P-selectin (^CT), ^CT + rPSGL-Ig mice, ^CT + Ab-PSGL-1 mice, WT + P-selectin (Psel) mice, and WT + P-selectin + rPSGL-Ig mice. WT + P-selectin mice had a 15% increase in thrombus mass above WT mice at day 2 and a 21% increase in TM above WT mice at day 6. n, Number of animals per group. Journal of Vascular Surgery  , DOI: ( /S (03) )

10 Fig 8 Fluorescence-activated cell scanning analysis with negative controls located on the left and study specimens on the right. Shown is a representative example of each group at day 2 (A) and day 6 (B) in ^CT + rPSGL-Ig, ^CT + Ab-PSGL-1 mice, WT + P-selectin, and WT + P-selectin + rPSGL-Ig. ^CT, Mice with high circulating levels of soluble P-selectin; WT, wild type. Journal of Vascular Surgery  , DOI: ( /S (03) )

11 Fig 8 Fluorescence-activated cell scanning analysis with negative controls located on the left and study specimens on the right. Shown is a representative example of each group at day 2 (A) and day 6 (B) in ^CT + rPSGL-Ig, ^CT + Ab-PSGL-1 mice, WT + P-selectin, and WT + P-selectin + rPSGL-Ig. ^CT, Mice with high circulating levels of soluble P-selectin; WT, wild type. Journal of Vascular Surgery  , DOI: ( /S (03) )

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