Presentation is loading. Please wait.

Presentation is loading. Please wait.

Figure S2 Gating strategy of AT2 cells based on EpCAM and MHCII

Published byThibaud Soucy Modified over 6 years ago

Similar presentations

Presentation on theme: "Figure S2 Gating strategy of AT2 cells based on EpCAM and MHCII"— Presentation transcript:

1 Figure S2 Gating strategy of AT2 cells based on EpCAM and MHCII
84.8 FSC SSC Live cells 86.6 7-AAD FSC singlet 95.5 FSC-Hight FSC-Width SSC singlet 92.0 SSC-Height SSC-Width CD45-CD31- cells 28.1 CD45,CD31-PE-Cy7 RatIgG2bκ,RatIgG2aκ-PE-Cy7 RatIgG2aκ-APC RatIgG2bκ-eFluor450 EpCAM-APC P1 89.3 P2 1.6 P3 2.6 MHCII-eFluor450 Figure S2 Gating strategy for isolation of AT2 cells based on EpCAM and MHCII expression Gating strategy for isolation of AT2 cells from single cell suspension of the lungs is demonstrated. Gating of each cell population was performed along the order of arrows. Dotted arrows indicate plots with relevant isotype controls.

Download ppt "Figure S2 Gating strategy of AT2 cells based on EpCAM and MHCII"

Similar presentations

Online supplementary material. Profound and persistent decrease of circulating dendritic cells is associated with ICU-acquired infection in patients with.

Online supplementary material. Profound and persistent decrease of circulating dendritic cells is associated with ICU-acquired infection in patients with.
IMMUNOPHENOTYPING OF HEMATOPOIETIC PROGENITOR CELLS BY MULTIPARAMETER FLOW CYTOMETRY.

IMMUNOPHENOTYPING OF HEMATOPOIETIC PROGENITOR CELLS BY MULTIPARAMETER FLOW CYTOMETRY.
A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.

A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.
Isotype control Supplementary Figure S1. Strategy for gating CD11b+CD14-CD33+CD40+ myeloid cells. Singlets were gated from live cells shown in forward.

Isotype control Supplementary Figure S1. Strategy for gating CD11b+CD14-CD33+CD40+ myeloid cells. Singlets were gated from live cells shown in forward.
Supplemental Figure 1 A. EpCAM Gating Strategy Top Row: Histogram showing unstained BT474 cells with low fluorescent intensity of the EpCAM specific PE.

Supplemental Figure 1 A. EpCAM Gating Strategy Top Row: Histogram showing unstained BT474 cells with low fluorescent intensity of the EpCAM specific PE.
Laser Flow Cytometry Forward Scatter indicates size Forward Scatter.

Laser Flow Cytometry Forward Scatter indicates size Forward Scatter.
Figure S1 (A) Analysis of purified myeloid DCs and T cells. Freshly isolated mDCs were processed for cytospins (A), stained with haematoxylin-eosin (x400.

Figure S1 (A) Analysis of purified myeloid DCs and T cells. Freshly isolated mDCs were processed for cytospins (A), stained with haematoxylin-eosin (x400.
CD44 PD-L1 FSC Human/Mouse Stromal Markers FSC DAPI FSC SSC FSC-W FSC-H SSC-W SSC-H Live cells Tumor cells CD44 - CD44 + Supplementary Figure S1. Gating.

CD44 PD-L1 FSC Human/Mouse Stromal Markers FSC DAPI FSC SSC FSC-W FSC-H SSC-W SSC-H Live cells Tumor cells CD44 - CD44 + Supplementary Figure S1. Gating.
AB C D E F G H Supplemental Fig. 1. Figure S1. MIF induces maturation of CD11b + but not CD8α + DCs in spleen from Mif -/- mice infected with T. gondii.

AB C D E F G H Supplemental Fig. 1. Figure S1. MIF induces maturation of CD11b + but not CD8α + DCs in spleen from Mif -/- mice infected with T. gondii.
Figure 1 WBC count (A), platelet count (B), MPV (C), platelet activation rate (D) and TGF-β1concentration (E) in the PF of women with endometriosis and.

Figure 1 WBC count (A), platelet count (B), MPV (C), platelet activation rate (D) and TGF-β1concentration (E) in the PF of women with endometriosis and.
SSC -A SLAN CD16 CD14 CD3-CD19-CD56 CD123 HLA-DR CD33

SSC -A SLAN CD16 CD14 CD3-CD19-CD56 CD123 HLA-DR CD33
D. Negative fraction after isolation of

D. Negative fraction after isolation of
Multiparametric flow cytometry of normal human bone marrow:

Multiparametric flow cytometry of normal human bone marrow:
mTECs produce tissue restricted antigens (TRAs)

mTECs produce tissue restricted antigens (TRAs)
Plot 4: A01 P0 CHO Count Events / μL % of This Plot % of All Mean FSC-A Mean SSC-A CV FSC-A CV SSC-A Median FSC-A Median SSC-A All 8,

Plot 4: A01 P0 CHO Count Events / μL % of This Plot % of All Mean FSC-A Mean SSC-A CV FSC-A CV SSC-A Median FSC-A Median SSC-A All 8,
Foxp3+ Regulatory T Cells and Natural Killer Cells Distinctly Infiltrate Primary Tumors and Draining Lymph Nodes in Pulmonary Adenocarcinoma  Thomas Schneider,

Foxp3+ Regulatory T Cells and Natural Killer Cells Distinctly Infiltrate Primary Tumors and Draining Lymph Nodes in Pulmonary Adenocarcinoma  Thomas Schneider,
Image 1 Detection of minimal residual disease (MRD) in consecutive bone marrow (BM) samples from a patient with relapse (A) and a patient still in remission.

Image 1 Detection of minimal residual disease (MRD) in consecutive bone marrow (BM) samples from a patient with relapse (A) and a patient still in remission.
Figure 2 Percentage of CD19+/CD34+ cells with decreased CD81 median fluorescence intensity (MFI;

Figure 2 Percentage of CD19+/CD34+ cells with decreased CD81 median fluorescence intensity (MFI;
Invest. Ophthalmol. Vis. Sci ;49(4): doi: /iovs Figure Legend:

Invest. Ophthalmol. Vis. Sci ;49(4): doi: /iovs Figure Legend:
Supplementary Figure 1 CD45 CD14 FSC CD123 HLA-DR CD11c Lineage

Supplementary Figure 1 CD45 CD14 FSC CD123 HLA-DR CD11c Lineage

Similar presentations

Ads by Google