1. Mitochondrial content reflects oocyte variability and fertilization outcome 
Teresa Almeida Santos, Ph.D., Shahy El Shourbagy, M.D., Justin C. St. John, Ph.D. 
Fertility and Sterility 
Volume 85, Issue 3, Pages (March 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
Real time PCR. Amplification of standards used to determine the number of mitochondria in a series of fertilized, unfertilized, and degenerate oocytes. Reaction conditions are described in the “Materials and Methods.” Legend: negative (blue); 1 in 10 dilution (pink); 1 in 100 dilution (yellow); 1 in 1,000 dilution (aqua); 1 in 10,000 dilution (purple); and 1 in 100,000 dilution (dark red).
Santos. Mitochondria in oocytes and embryos. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Melt curve analysis. Melt curves were constructed following each reaction to determine whether mispriming had occurred in any reactions by performing Sybr Green fluorescence reactions at temperatures between 55–93°C. Any reactions perceived to contain mispriming were repeated to eliminate doubt.
Santos. Mitochondria in oocytes and embryos. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions