1. Blood Prof. Ji-Cheng Li (Zhejiang University School of Medicine)

2. red blood cell-erythrocyte white blood cell-leukocyte platelets
1.       Components:
---formed elements: 45%
red blood cell-erythrocyte
white blood cell-leukocyte
platelets
plasma
platelets
leukocyte
erythrocyte

3. ---plasma: 55%, PH ,
90% water
contain:
plasma protein (albumin, globulin, fibrinogen),
lipidprotein
enzymes
hormone
vitamin
inorganic salt
products of metabolism
serum: plasma without fibrinogen

4. Blood smear: Wright or Giemsa stain methods

5. 1.   Cells

6. 1) erythrocyte, red blood cell
---normal number:
( )X1012/L in male; ( )X1012/L in female
---structure:
biconcave discs in shape,
um in diameter, 1-2um thick
no nucleus and organella
filled with hemoglobin (Hb)

7. -normal Hb :
g/L in male
g/L in female
-Function:
combine and transfer Oxygen (O2) and Carbon dioxide(CO2)

10. reticulocyte: immature cell
remained ribosome: delicate network structure when stained with brilliant cresyl blue
0.5-1% of total erythrocyte population in adult
3-6% in neonate
---life span: 120 days

11. 2) leukocyte ---a group of large cells with nucleus
---involve in defense and immune reaction
---normal number: (4.0-10) X109/L
---classification:
granulocytes: /neutrophil
/eosinophil
/basophil
agranulocytes:
/lymphocyte
/monocyte

12. ①     neutrophil:
50-70% of total leukocyte population
---structure:
LM:
round, 10-12um in diameter
rob-liked or polymorphous nucleus, 2-5 lobes
fine neutrophilic granules
pink cytoplasm

13. EM:
specific granule:
80%
small, um
ovoid or irregular in shape
contain: lysozyme, phagocytin (defensin)
azurophilic granule:
20%
large, round or ovoid , um
electron dense
contain: acid phosphatase, peroxidase, ect.

14. ---function: phagocytose bacterium:
specific g.-kill B
azurophilic g.- digest B
---life span: in blood 6-8 hours, in CT 2-3 days

16. Neutrophilic granulocyte

17. ②     eosinophil:
0.5-3% of total leukocyte population
LM:
round,10-15 um in diameter
2 lobes nucleus
rough bright red granules: acidophilic granules

18. EM: granules:
round or ovoid
with cube-liked electron dense crystal
contain:
-ACPase
-histaminase
-peroxidase

19. ---function:
counteract the infection of parasite
reduce allergic reaction
---life span: in blood 6-8 hour, in CT 8-12 days

21. Eosinophilic granulocyte
致密结晶体

22. ③ basophil: 0-1% of leukocyte population LM: less, round 10-12 um
lobe, “S” shaped or irregular nucleus
basophilic g.: contains heparin, histamine and acidophil chemotactic factor
cytoplasm: contain leukotriene
---function: involve in allergic reaction
---life span: days

24. Basophilic granulocyte

25. ④ monocyte: 3-8% of total leukocyte population LM:
large round, 14-20um
nucleus appear as kidney, horse-shoe or ovoid in shape
cytoplasm: gray-blue in color, contain azurophilic g.

26. azurophilic granule: peroxidase, ACPase, lysozyme
---function: actively mobile and chemotaxis
---life span: in blood 1-5 days, in CT which become into macrophage

33. ⑤ lymphocyte:
20-30% of total
leukocyte population
LM:
round, small LC 6-8 um,
medium-sized LC 9-12 um,
large LC um in diameter
round nucleus with indentation, chromatin appears as spot-liked and electron-dense
cytoplasm: /basophilic:bright blue in color
/less
/azurophilic granule

34. EM: /free ribosome
/mito.
/RER
---classification:
TC: thymus dependent lymphocyte, 75%, involve in cellular immune reaction and regulate immune response
BC: bone marrow dependent lymphocyte, 10-15%, become into plasma cell, involve in humoral immune response
large granular cell: KC (killer cell) and NKC( nature killer cell), 10%
---function: involve in immune response

36. Lymphocyte

40. division

41. cytoplasmic fragment of megakaryocyte in bone marrow
3) blood platelet:
cytoplasmic fragment of megakaryocyte in bone marrow
---normal number: X109/L
---structure:
LM:
disc-liked, 2-4um → irregular in shape
Stimulation
in groups
Granulomere
hyalomere

42. ---function:
involve in clotting and stopping the bleed
protecting endothelium and take part in repairing of endothelium

43. 血小板（EM）
Blood platelet
特殊颗粒

48. Question

49. ???

50. TEM
neutrophil

51. ???

52. TEM
monocyte

53. ???

54. Eosinophilic granulocyte
TEM
Eosinophilic granulocyte

55. ???

56. TEM
Lymphocyte

57. ???

58. Basophilic granulocyte

59. When hemoglobin of erythrocyte escapes into the plasma, the outward passage of hemoglobin is called
A. anemia
B. microcyte
C. macrocyte
D. hemolysis
E. agglutination √

60. During development the platelets are derived from the azurophilic granules of
A. macrophage
B. megakaryocyte
C. lymphoblast
D. monoblast
E. erythroblast √

61. The blood components are formed by plasma and formed elements
The blood components are formed by plasma and formed elements. The later includes _______________________, ____________________________ and __________________.
red blood cell-erythrocyte
white blood cell-leukocyte
platelets

62. The cytoplasm of erythrocyte contains rich ______________ which combined with __________________ or __________________. The immature erythrocyte is ____________________.
hemoglobin (Hb)
Oxygen (O2)
Carbon dioxide(CO2)
reticulocyte

63. Thanks!