Presentation is loading. Please wait.

Presentation is loading. Please wait.

Silver acetate coating promotes early vascularization of Dacron vascular grafts without inducing host tissue inflammation  Pascal Jeanmonod, MD, Matthias.

Published bySylvia Sutton Modified over 6 years ago

Similar presentations

Presentation on theme: "Silver acetate coating promotes early vascularization of Dacron vascular grafts without inducing host tissue inflammation  Pascal Jeanmonod, MD, Matthias."— Presentation transcript:

1 Silver acetate coating promotes early vascularization of Dacron vascular grafts without inducing host tissue inflammation  Pascal Jeanmonod, MD, Matthias W. Laschke, PhD, Nils Gola, Maximilian von Heesen, MD, Matthias Glanemann, MD, Stefan Dold, MD, Michael D. Menger, MD, Mohammed R. Moussavian, MD  Journal of Vascular Surgery  Volume 58, Issue 6, Pages (December 2013) DOI: /j.jvs Copyright © 2013 Society for Vascular Surgery Terms and Conditions

2 Fig 1 A, Stereomicroscopic overview of a Dacron Silver vascular graft (2 × 2 × 0.35 mm) directly after implantation onto the striated muscle tissue in the dorsal skinfold chamber of a C57BL/6 mouse. Note that the larger microvessels of the chamber (arterioles and venules of the striated muscle and subcutaneous tissue) can be identified without the use of high-resolution intravital fluorescence microscopy. B, The density of newly formed blood-perfused microvessels growing into the implant was measured in eight different microvascular regions-of-interest (ROIs; red boxes; size: 0.4 mm²), which were directly located at the interface between the prosthetic vascular graft and the surrounding host tissue. In addition, four different ROIs in the border zone of the implant, (ie, in close vicinity to the material [blue boxes]), leukocyte-endothelial cell interaction, and microhemodynamics were measured in postcapillary and collecting venules of the host tissue. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

3 Fig 2 Intravital fluorescent microscopic images of the border of Dacron (A) and Dacron Silver vascular grafts (B) at day 14 after implantation into the dorsal skinfold chamber of C57BL/6 mice. Blue light epi-illumination with contrast enhancement by 5% fluorescein isothiocyanate (FITC)-labeled dextran 150,000 i.v. C, Functional capillary density (cm/cm²) within the border of Dacron (white bars; n = 8) and Dacron Silver vascular grafts (black bars; n = 8), as assessed by intravital fluorescence microscopy and computer-assisted image analysis. Means ± standard error of the mean (SEM). *P < .05 vs Dacron at corresponding time points. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

4 Fig 3 Rolling leukocytes (A, min−1) and adherent leukocytes (B, mm−2) in postcapillary and collecting venules within the border zones of Dacron (white bars; n = 8) and Dacron Silver vascular grafts (black bars; n = 8), as assessed by intravital fluorescence microscopy and computer-assisted image analysis. Means ± standard error of the mean (SEM). *P < .05 vs Dacron at corresponding time points. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

5 Fig 4 Hematoxylin-eosin stained cross sections of the border zone of a Dacron Silver vascular graft (asterisk) at day 14 after implantation onto the striated muscle tissue (A, arrows) within the dorsal skinfold chamber of a C57BL/6 mouse. Higher magnification (B) of the interface between the implant and the surrounding tissue (A, black window) shows the dense vascularization and ingrowth of a fibrovascular tissue in between the Dacron fibers. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

6 Fig 5 Histologic cross-sections of the border zones of a Dacron (A, C, E) and a Dacron Silver vascular graft (B, D, F) at day 14 after implantation onto the striated muscle tissue within the dorsal skinfold chambers of C57BL/6 mice. The sections were stained with Sirius red (A and B) for the visualization of collagen fibers or antibodies against proliferating cell nuclear antigen (PCNA) (C and D) and caspase-3 (casp-3) (E and F) for the detection of proliferating and apoptotic cells. PCNA-positive cells (G, %) and casp-3-positive cells (H, %) in the border zones of Dacron (white bars; n = 8) and Dacron Silver vascular grafts (black bars; n = 8) at day 14. Means ± standard error of the mean (SEM). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Download ppt "Silver acetate coating promotes early vascularization of Dacron vascular grafts without inducing host tissue inflammation  Pascal Jeanmonod, MD, Matthias."

Similar presentations

Decline of Tumor Vascular Function as Assessed by Dynamic Contrast-Enhanced Magnetic Resonance Imaging Is Associated With Poor Responses to Radiation.

Decline of Tumor Vascular Function as Assessed by Dynamic Contrast-Enhanced Magnetic Resonance Imaging Is Associated With Poor Responses to Radiation.
Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 
Liposuction-derived human fat used for vascular graft sodding contains endothelial cells and not mesothelial cells as the major cell type  Stuart K. Williams,

Liposuction-derived human fat used for vascular graft sodding contains endothelial cells and not mesothelial cells as the major cell type  Stuart K. Williams,
Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice  Daniël Eefting,

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice  Daniël Eefting,
Selective cyclo-oxygenase-2 inhibition induces regression of autologous endometrial grafts by down-regulation of vascular endothelial growth factor-mediated.

Selective cyclo-oxygenase-2 inhibition induces regression of autologous endometrial grafts by down-regulation of vascular endothelial growth factor-mediated.
Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,
Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps  Rahmi Oklu,

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps  Rahmi Oklu,
In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,

In vivo electroporation of constitutively expressed HIF-1α plasmid DNA improves neovascularization in a mouse model of limb ischemia  Geoffrey O. Ouma,
Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD 

Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD 
Implantation of amniotic membrane as a vascular substitute in the external jugular vein of juvenile sheep  Habibollah Peirovi, MD, Navid Rezvani, MD,

Implantation of amniotic membrane as a vascular substitute in the external jugular vein of juvenile sheep  Habibollah Peirovi, MD, Navid Rezvani, MD,
Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,

Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,
Increased endogenous angiogenic response and hypoxia-inducible factor-1α in human critical limb ischemia  Teik K. Ho, MRCS, Vineeth Rajkumar, MSc, Markella.

Increased endogenous angiogenic response and hypoxia-inducible factor-1α in human critical limb ischemia  Teik K. Ho, MRCS, Vineeth Rajkumar, MSc, Markella.
The therapeutic effect of vascular endothelial growth factor gene- or heme oxygenase-1 gene-modified endothelial progenitor cells on neovascularization.

The therapeutic effect of vascular endothelial growth factor gene- or heme oxygenase-1 gene-modified endothelial progenitor cells on neovascularization.
Effect of tissue plasminogen activator on vascular smooth muscle cells

Effect of tissue plasminogen activator on vascular smooth muscle cells
Hyperphosphatemia induces protective autophagy in endothelial cells through the inhibition of Akt/mTOR signaling  Yu-Juei Hsu, MD, PhD, Shih-Che Hsu,

Hyperphosphatemia induces protective autophagy in endothelial cells through the inhibition of Akt/mTOR signaling  Yu-Juei Hsu, MD, PhD, Shih-Che Hsu,
Increased 18F-fluorodeoxyglucose uptake in abdominal aortic aneurysms in positron emission/computed tomography is associated with inflammation, aortic.

Increased 18F-fluorodeoxyglucose uptake in abdominal aortic aneurysms in positron emission/computed tomography is associated with inflammation, aortic.
Celiprolol reduces the intimal thickening of autogenous vein grafts via an enhancement of nitric oxide function through an inhibition of superoxide production 

Celiprolol reduces the intimal thickening of autogenous vein grafts via an enhancement of nitric oxide function through an inhibition of superoxide production 
Angiogenic effects of stromal cell-derived factor-1 (SDF-1/CXCL12) variants in vitro and the in vivo expressions of CXCL12 variants and CXCR4 in human.

Angiogenic effects of stromal cell-derived factor-1 (SDF-1/CXCL12) variants in vitro and the in vivo expressions of CXCL12 variants and CXCR4 in human.
VEGF Gene Delivery to Muscle

VEGF Gene Delivery to Muscle
Infection of vascular prostheses caused by bacterial biofilms

Infection of vascular prostheses caused by bacterial biofilms

Similar presentations

Ads by Google