1. 1 3 5 DMSO Skin Liver Kidney Lung Spleen Bar : 500 mm
Inoue et al., Figure SI15. 1 3 5
DMSO
Skin
Liver
Kidney
Lung
Spleen
Bar : 500 mm
Figure SI15. The fluorescent image of back skin and tissue sections prepared from compounds-treated mice.
Each compound (1, 3, and 5: 1 mg/kg, DMSO: 0.1% v/v) was intravenously administrated into naive 6-weeks female BALB/c nude mice (Oriental Yeast, Tokyo, Japan). These mice were sacrificed at 30 minutes after administration and then skin, liver, kidney, heart, lung, and spleen were resected. Frozen sections were mounted with VECTASHIELD Mounting Medium with DAPI (Vector Laboratory, Burlingame, CA, USA) and visualized cellular nuclear and fluorescein –conjugated PI polyamides under a BZ-X710 microscope (Keyence Corporation, Osaka, Japan). Bars indicate indicate 500 mm.