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The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro  Carol J. Sawmiller, MD, Richard J. Powell, MD,

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Presentation on theme: "The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro  Carol J. Sawmiller, MD, Richard J. Powell, MD,"— Presentation transcript:

1 The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro  Carol J. Sawmiller, MD, Richard J. Powell, MD, Mohammed Quader, MD, Stanley J. Dudrick, MD, Bauer E. Sumpio, MD, PhD  Journal of Vascular Surgery  Volume 27, Issue 6, Pages (June 1998) DOI: /S (98) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Comparison of cell proliferation after exposure to three different contrast agents. Cells were exposed to full strength contrast for 10 minutes. One representative experiment is shown. Data are mean ± SEM. *p < 0.05 compared with control. Top graph, EC proliferation. Conray inhibited proliferation to greater degree than Optiray or Isovue. (p < 0.05). Bottom graph, SMC proliferation. Exposure to all contrast agents significantly decreased proliferation at day 1 and 3, with exposure to Optiray causing greatest effect. (p < 0.05 compared with Conray and Isovue.) Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Lactate dehydrogenase (LDH) activity after 10-minute exposure to three different contrast agents. Top graph, LDH activity in EC media. Bottom graph, LDH activity in SMC media. Exposure to contrast did not increase LDH activity. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Cell proliferation after varying exposure times. Cells were exposed to Conray for periods of 1, 3, 5, 10, or 20 minutes. Data are mean ± SEM. *p < 0.05 compared with control. Top graph, EC proliferation. Proliferation was reduced as exposure time to contrast was prolonged. Bottom graph, SMC proliferation. Similar pattern was observed in SMC proliferation, with significance at an exposure time of 10 minutes or longer. (p < 0.05). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 Cell proliferation after exposure to dilutions of Conray contrast. Data are mean ± SEM. *p < 0.05 compared with control. Top graph, EC proliferation. Full strength contrast significantly reduces cell proliferation compared with 1/2 strength, which significantly reduces proliferation compared with 1/4 strength at 24 hours after exposure. (p < 0.05 for all groups). Bottom graph, SMC proliferation. SMC proliferation is significantly reduced after exposure to full strength contrast. (p < 0.05). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 5 Comparison of effect of Conray and varying osmolar solutions of mannitol on EC. Cells were exposed to Conray or mannitol solution for 10 minutes. Data are mean ± SEM. *p < 0.05 compared with control. Top graph, EC were exposed to Conray and an isosmolar solution of mannitol. , EC were exposed to 1400, 700, or 350 mOsm/kg mannitol. Effect on proliferation increased in proportion to increased osmolality. (p < 0.05). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 6 Comparison of effect of Optiray and varying osmolar solutions of mannitol on SMC. Data are mean ± SEM. *p < 0.05 compared with control. Top graph, SMC were exposed to Optiray, isosmolar,and hyperosmolar solution of mannitol. Optiray reduced cell proliferation significantly more than both isosmolar and hyperosmolar solutions. (p < 0.05). Bottom graph, SMC were exposed to 1400, 700, or 350 mOsm/kg mannitol. SMC proliferation was not dependent on osmolality. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8 Fig. 7 EC. A, EC immediately after washing or contrast exposure. B, EC after 1-hour incubation period. C, EC after 24 hours. D, EC after 72-hour incubation period. Photomicrograph ×200 magnification. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

9 Fig. 8 SMC. A, SMC immediately after washing or contrast exposure. B, SMC 1 hour after washing or contrast exposure. C, SMC after 24-hour incubation. D, SMC after 72 hours. Photomicrograph ×200 magnification. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

10 Fig. 9 EC 1 hour after 10-minute exposure to mannitol. A, Control EC demonstrate cobblestone growth pattern typical of confluent EC in culture. B, EC exposed to 1400 mOsm/kg mannitol. Many injured, spherical cells are seen. C, EC exposed to 700 mOsm/kg mannitol. Cell shrinkage is evident, but not as extensive as seen with 1400 mOsm/kg mannitol. D, EC exposed to 350 mOsm/kg mannitol. Minimal cell injury is seen. Photomicrograph ×200 magnification. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

11 Fig. 10 SMC 1 hour after 10-minute exposure to mannitol. A, Control SMC demonstrate confluent, swirled growth pattern. B, SMC exposed to 1400 mOsm/kg mannitol. Few damaged cells are evident with little cell lifting from the matrix. C, SMC exposed to 700 mOsm/kg mannitol. Cells appear normal with no evidence of injury. D, SMC exposed to 350 mOsm/kg mannitol. Cells also appear normal with no shrinkage. Photomicrograph ×200 magnification. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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