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Dendritic cells and alveolar macrophages mediate IL-13–induced airway inflammation and chemokine production  Margaret Crapster-Pregont, BS, Janice Yeo,

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Presentation on theme: "Dendritic cells and alveolar macrophages mediate IL-13–induced airway inflammation and chemokine production  Margaret Crapster-Pregont, BS, Janice Yeo,"— Presentation transcript:

1 Dendritic cells and alveolar macrophages mediate IL-13–induced airway inflammation and chemokine production  Margaret Crapster-Pregont, BS, Janice Yeo, BS, Raquel L. Sanchez, BS, Douglas A. Kuperman, PhD  Journal of Allergy and Clinical Immunology  Volume 129, Issue 6, Pages e3 (June 2012) DOI: /j.jaci Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 IFN-γ inhibits IL-13–induced airway inflammation and mucus metaplasia. A, Cell types and numbers retrieved in BAL fluid from PBS-treated (open bars), IL-13–treated (black bars), and IL-13/IFN-γ–treated mice (shaded bars). Eos, Eosinophils; Lymphs, lymphocytes; Macs, macrophages. B-D, Representative images of mucus metaplasia in PBS-treated (Fig 1, B), IL-13–treated (Fig 1, C), and IL-13/IFN-γ–treated (Fig 1, D) wild-type mice (n = 6 mice per group). ∗P ≤ .05 versus PBS. †P ≤ .05 versus IL-13. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 IFN-γ inhibits a subset of IL-13–induced chemokines. Levels of CCL11 (A), CCL24 (B), CCL22 (C), CCL17 (D), CCL8 (E), and CCL2 (F) in BAL fluid from PBS-treated (open bars), IL-13–treated (black bars), and IL-13/IFN-γ–treated wild-type mice (shaded bars) are shown (n = 6 mice per group). ∗P ≤ .05 versus PBS. †P ≤ .05 versus IL-13. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Dendritic cells and alveolar macrophages coexpress IL-13R and IFN-γR subunits. Numbers of IL-4Rα+/IFN-γRα+ cells (A) and their proportions that express CD11b and CD11c (B) from the lungs of PBS-treated (open bars) and IL-13–treated (shaded bars) wild-type mice in MHC-II+ cell–depleted flow-through and MHC-II+ cell–enriched bound fractions are shown (n = 6 mice per group). ∗P ≤ .05 versus flow-through. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Dendritic cell and alveolar macrophage targeting protects mice from IL-13-induced airway inflammation and chemokine production. A, Gene expression levels of DC-SIGN in whole lung. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase. B-D, Numbers of alveolar macrophages (Macs; Fig 4, B); numbers of eosinophils (Eos), lymphocytes (Lymphs), and neutrophils (Neuts; Fig 4, C); and levels of chemokines (Fig 4, D) in BAL fluid from CD11c-DTR mice (n = 6 mice per group). ∗P ≤ .05 versus PBS. †P ≤ .05 versus IL-13. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 Dendritic cell targeting protects mice from IL-13–induced airway inflammation and a subset of IL-13–induced chemokines. A, Gene expression levels of DC-SIGN in whole lung. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase. B-D, Numbers of alveolar macrophages (Macs; Fig 5, B); numbers of eosinophils (Eos), lymphocytes (Lymphs), and neutrophils (Neuts; Fig 5, C); and levels of chemokines (Fig 5, D) in BAL fluid from CD11b-DTR mice (n = 6 mice per group). ∗P ≤ .05 versus PBS/PBS. †P ≤ .05 versus PBS/IL-13. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E1 Effects of DT treatment on baseline levels of dendritic cells and macrophages in whole lung. A and B, Percentages of CD11b+/CD11c+ cells (Fig E1, A) and CD204+/(F4/80)+ cells (Fig E1, B) detected in whole lungs of CD11c-DTR mice. C and D, Percentages of CD11b+/CD11c+ cells (Fig E1, C) and CD204+/(F4/80)+ cells (Fig E1, D) detected in whole lungs of CD11b-DTR mice. Representative plots are shown from 4 mice per group. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E2 Dendritic cell and alveolar macrophage targeting does not protect mice from IL-13–induced AHR and mucus metaplasia. AHR to acetylcholine injection (A) and representative images showing mucus metaplasia (B) in the lungs of CD11c-DTR mice (n = 6 mice per group.) ∗P ≤ .05 versus PBS. Journal of Allergy and Clinical Immunology  , e3DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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