1. Chapter 17
From gene to protein

2. One gene/One polypeptide

3. Define transcription and translation Compare Prokaryotic and Eukaryotic cells video

4. (21 different AA’s)
mRNA code
Ave. protein is 400AA long

5. DNA= triplets
RNA= codons
5’ to 3’
Pre-mRNA (primary transcript)

6. Promoter: TATA Box
Transcription factors
RNA poly- merase
Transcription initiation complex
Movie

7. Finishing the pre-mRNA
Introns: (intruding) spliced out
Exons: kept, will be Expressed in the cell

8. video snRNP’s identify introns Introns are cut out at a Spliceosome
Final mRNA has only genes that will be tran-scribed in cell no “junk genes”
video

9. Translation Read 5’ to 3’ The production of polypeptides on a Ribosome
tRNA, transfer RNA carries in the amino acids to the ribosome (transfers the A.A)
Read 5’ to 3’

12. A P E: Video Movie Aminoacyl-tRNA synthetase
Peptidyl tRNA binding site
Exit
Video
Movie

14. Termination of the translation

15. Translation of many polypeptide chains at once

16. The signal mechanism: signal peptides
Free or bound ribosomes?

17. Coupled transcription and translation

18. Mutations: Look at the result of just one base difference out of 574 Amino acids (1722 bases)

19. The wrong protein
Nothing formed

20. Repairing mistakes

21. Summary