Presentation is loading. Please wait.

Presentation is loading. Please wait.

Decellularized heart valve as a scaffold for in vivo recellularization: Deleterious effects of granulocyte colony-stimulating factor  Francis Juthier,

Published byArabella Lamb Modified over 5 years ago

Similar presentations

Presentation on theme: "Decellularized heart valve as a scaffold for in vivo recellularization: Deleterious effects of granulocyte colony-stimulating factor  Francis Juthier,"— Presentation transcript:

1 Decellularized heart valve as a scaffold for in vivo recellularization: Deleterious effects of granulocyte colony-stimulating factor  Francis Juthier, MD, André Vincentelli, MD, PhD, Julien Gaudric, MD, Delphine Corseaux, PhD, Olivier Fouquet, MD, Christine Calet, MD, Thierry Le Tourneau, MD, PhD, Valérie Soenen, BS, Christophe Zawadzki, BS, Olivier Fabre, MD, Sophie Susen, MD, Alain Prat, MD, Brigitte Jude, MD, PhD  The Journal of Thoracic and Cardiovascular Surgery  Volume 131, Issue 4, Pages (April 2006) DOI: /j.jtcvs Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

2 Figure 1 Kinetics of white blood cell counts in lambs after subcutaneous injection of recombinant human G-CSF (10 μg/kg) for 7 days. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

3 Figure 2 Results of the decellularization process (hematoxylin, eosin, and safran staining). Before and after decellularization: A, native valve conduit; B, leaflet. A complete decellularization of the aortic wall and of the leaflets without destruction of the fibrous structure is shown. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

4 Figure 3 A and B, Representative aspects of normal aortography (A) at 16 weeks and perioperative aspect of the graft in the thoracic descending aorta (B). C and D, Specific aspect of the 16 weeks after implantation aortography (C) and of the explanted valve (D) in the animal that underwent complete valve thrombosis (xenograft group). Arrows indicate site of the graft implantation. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

5 Figure 4 Macroscopic examination at 16 weeks. A, Control group; B, G-CSF group; C, Xenograft group. In the xenograft and the G-CSF groups, but not in the control group, gross observation showed calcifications of the leaflets and of the aortic wall, leaflet thickening, and shrinkage at 16 weeks. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

6 Figure 5 Representative histologic aspects in the grafted aortic wall at 16 weeks in the 3 groups (top row, control group; middle row, G-CSF group; bottom row, xenograft group). A, Adventitia (Masson trichrome staining): collagen fibers and limited infiltration by inflammatory cells were visible in the control group. Inflammatory cells were more prominent in the G-CSF and xenograft groups than in the control group. B (anti-VWF antibody), Adventitial neovascularization (arrows) was limited in the control group and abundant in the G-CSF and xenograft groups. C (Masson trichrome staining), Neointimal proliferation tended to be more important in the G-CSF and xenograft groups than in the control group. D (anti-α-actin staining), Evidence of strong α-actin staining in the neointimal layer in the 3 groups. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

7 Figure 6 Orcein, von Kossa, and immunochemical staining with anti-VWF antibody in the 3 groups and representative aspects of the leaflets are shown (top row, control group; middle row, G-CSF group; bottom row, xenograft group). A (orcein staining), Elastic fibers remained parallel in the control group and were disrupted and disorganized in the xenograft and G-CSF groups. B (Von Kossa staining), Evidence of heavy calcifications in the aortic wall in the G-CSF and xenograft groups and not in the control group. C (anti-VWF antibody), The luminal surface of the aortic wall was covered by a continuous layer of endothelial cells in the control group and by a disrupted layer (arrows) in the G-CSF and xenograft groups. D (Masson trichrome staining), Representative aspects of the leaflets in the 3 groups, with calcifications in the G-CSF and xenograft groups. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

8 Figure 7 Time course of the intima/media ratio in the 3 groups.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

9 Figure 8 Results of fluorescence in situ hybridization on the cells eluted from the graft (A), a positive control on ovine blood (B), and a negative control on porcine blood (C). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2006 The American Association for Thoracic Surgery Terms and Conditions

Download ppt "Decellularized heart valve as a scaffold for in vivo recellularization: Deleterious effects of granulocyte colony-stimulating factor  Francis Juthier,"

Similar presentations

In vitro tissue engineering of a cardiac graft using a degradable scaffold with an extracellular matrix–like topography  Osamu Ishii, MD, Michael Shin,

In vitro tissue engineering of a cardiac graft using a degradable scaffold with an extracellular matrix–like topography  Osamu Ishii, MD, Michael Shin,
Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 
Max B. Mitchell, MD  The Journal of Thoracic and Cardiovascular Surgery 

Max B. Mitchell, MD  The Journal of Thoracic and Cardiovascular Surgery 
Manuel J. Antunes, MD, PhD, DSc 

Manuel J. Antunes, MD, PhD, DSc 
New engineering treatment of bovine pericardium confers outstanding resistance to calcification in mitral and pulmonary implantations in a juvenile sheep.

New engineering treatment of bovine pericardium confers outstanding resistance to calcification in mitral and pulmonary implantations in a juvenile sheep.
In vivo autologous recellularization of a tissue-engineered heart valve: Are bone marrow mesenchymal stem cells the best candidates?  Andre Vincentelli,

In vivo autologous recellularization of a tissue-engineered heart valve: Are bone marrow mesenchymal stem cells the best candidates?  Andre Vincentelli,
Tracheal regeneration: Myth or fact?

Tracheal regeneration: Myth or fact?
Cardiac xenotransplantation technology provides materials for improved bioprosthetic heart valves  Christopher G.A. McGregor, MB, FRCS, MD(Hons), Alain.

Cardiac xenotransplantation technology provides materials for improved bioprosthetic heart valves  Christopher G.A. McGregor, MB, FRCS, MD(Hons), Alain.
Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential  Lorenzo Boni, MD,

Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential  Lorenzo Boni, MD,
Antidonor humoral transfer induces transplant arteriosclerosis in aortic and cardiac graft models in rats  Bassam Alkhatib, MD, Caroline Freguin-Bouilland,

Antidonor humoral transfer induces transplant arteriosclerosis in aortic and cardiac graft models in rats  Bassam Alkhatib, MD, Caroline Freguin-Bouilland,
Prevention of the second stage of epithelial loss is a potential novel treatment for bronchiolitis obliterans  Yunge Zhao, MD, PhD, John F. Steidle, BA,

Prevention of the second stage of epithelial loss is a potential novel treatment for bronchiolitis obliterans  Yunge Zhao, MD, PhD, John F. Steidle, BA,
Biomatrix/Polymer Composite Material for Heart Valve Tissue Engineering  Christof Stamm, MD, Amir Khosravi, MD, Niels Grabow, MS, Kathleen Schmohl, PhD,

Biomatrix/Polymer Composite Material for Heart Valve Tissue Engineering  Christof Stamm, MD, Amir Khosravi, MD, Niels Grabow, MS, Kathleen Schmohl, PhD,
Experimental noninferiority trial of synthetic small-caliber biodegradable versus stable vascular grafts  Damiano Mugnai, MD, Jean-Christophe Tille, MD,

Experimental noninferiority trial of synthetic small-caliber biodegradable versus stable vascular grafts  Damiano Mugnai, MD, Jean-Christophe Tille, MD,
Tracheal regeneration: Evidence of bone marrow mesenchymal stem cell involvement  Agathe Seguin, MD, PhD, Sonia Baccari, MD, Muriel Holder-Espinasse, MD,

Tracheal regeneration: Evidence of bone marrow mesenchymal stem cell involvement  Agathe Seguin, MD, PhD, Sonia Baccari, MD, Muriel Holder-Espinasse, MD,
Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches  Eric J. Lehr, MD, PhD, Gina R. Rayat, PhD, Brian Chiu, MD, Thomas.

Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches  Eric J. Lehr, MD, PhD, Gina R. Rayat, PhD, Brian Chiu, MD, Thomas.
Tissue-engineered stent-graft integrates with aortic wall by recruiting host tissue into graft scaffold  Mugiho Takeuchi, MD, Toru Kuratani, MD, PhD,

Tissue-engineered stent-graft integrates with aortic wall by recruiting host tissue into graft scaffold  Mugiho Takeuchi, MD, Toru Kuratani, MD, PhD,
Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 
Dissection of a dilated autograft root

Dissection of a dilated autograft root
Intraoperative identification of esophageal sentinel lymph nodes with near-infrared fluorescence imaging  Cherie P. Parungo, MD, Shunsuke Ohnishi, MD,

Intraoperative identification of esophageal sentinel lymph nodes with near-infrared fluorescence imaging  Cherie P. Parungo, MD, Shunsuke Ohnishi, MD,
Constructive Remodeling of CorMatrix Extracellular Matrix After Aortic Root Repair in a 90-Year-Old Woman  Frank N. Slachman, MD  The Annals of Thoracic.

Constructive Remodeling of CorMatrix Extracellular Matrix After Aortic Root Repair in a 90-Year-Old Woman  Frank N. Slachman, MD  The Annals of Thoracic.

Similar presentations

Ads by Google