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Atherogenic effects of chlamydia pneumoniae: refuting the innocent bystander hypothesis  Craig H Selzman, MD, Mihai G Netea, MD, PhD, Michael A Zimmerman,

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Presentation on theme: "Atherogenic effects of chlamydia pneumoniae: refuting the innocent bystander hypothesis  Craig H Selzman, MD, Mihai G Netea, MD, PhD, Michael A Zimmerman,"— Presentation transcript:

1 Atherogenic effects of chlamydia pneumoniae: refuting the innocent bystander hypothesis 
Craig H Selzman, MD, Mihai G Netea, MD, PhD, Michael A Zimmerman, MD, Adriana Weinberg, MD, Leonid L Reznikov, MD, PhD, Frederick L Grover, MD, Charles A Dinarello, MD  The Journal of Thoracic and Cardiovascular Surgery  Volume 126, Issue 3, Pages (September 2003) DOI: /S (03)

2 Figure 1 Influence of Chlamydia pneumoniae infection on human vascular smooth muscle cell (VSMC) proliferation. A, After 24 hours, VSMC proliferation as measured by methoxy-tetrazolium salt (MTS) assay and reported as optical density (*vs control, P < .02). B, After inoculation with 250 inclusion-forming units (IFU)/mL of C. pneumoniae, VSMC proliferation as measured by cell numbers at 24 and 48 hours (*vs control, P < .02). Both methods demonstrated a positive influence of C. pneumoniae on VSMC growth. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

3 Figure 2 Influence of C. pneumoniae infection on human VSMC expression of proliferating cell nuclear antigen (PCNA). Twenty-four hours after inoculation, VSMCs were stained immunohistochemically for PCNA (green), nuclei (blue), and membrane glycoproteins (red). The top panels (A) incorporate all 3 stains. The lower panels (B) filter out the nuclear stain to demonstrate the intranuclear presence of PCNA. Compared with control VSMC, C. pneumoniae infection increased PCNA expression. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

4 Figure 3 Influence of C. pneumoniae infection on human VSMC expression of p53. Electromobility gel shift assays were performed 1 hour after VSMC stimulation. Band densities were quantified over 3 successive experiments using 3 separate donor VSMCs. Compared with control VSMC, C. pneumoniae inoculation resulted in decreased p53 DNA binding (*P < .02). This effect was partly mitigated by concomitant administration of the macrolide antibiotic azithromycin (†vs C. pneumoniae, P < .02). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

5 Figure 4 Influence of C. pneumoniae infection on human VSMC production of interleukin (IL)-6 and IL-8. Compared with control, C. pneumoniae induces both IL-6 and IL-8 production (*P < .02). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /S (03) )

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