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Published byGustav Olofsson Modified over 6 years ago
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Use of frozen-thawed testicular sperm for intracytoplasmic sperm injection
Wolfgang Küpker, M.D., Ph.D., Peter N Schlegel, M.D., Safaa Al-Hasani, Ph.D., Paolo Fornara, M.D., Ph.D., Rainer Johannisson, Ph.D., Jörg Sandmann, M.D., Thilo Schill, M.D., Monika Bals-Pratsch, M.D., Michael Ludwig, M.D., Klaus Diedrich, M.D., Ph.D. Fertility and Sterility Volume 73, Issue 3, Pages (March 2000) DOI: /S (99)
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Figure 1 Open testicular biopsy (two biopsy sites).
Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility , DOI: ( /S (99) )
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Figure 2 Extracted testicular tissue before freezing.
Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility , DOI: ( /S (99) )
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Figure 3 Sertoli cell–only pattern with focal spermatogenesis. Toluidine blue/pyronine stain. Original magnification, ×1,000). Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility , DOI: ( /S (99) )
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Figure 4 Protocol for freezing and thawing of testicular tissue.
Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility , DOI: ( /S (99) )
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