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Wolfgang Küpker, M. D. , Ph. D. , Peter N Schlegel, M. D

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Presentation on theme: "Wolfgang Küpker, M. D. , Ph. D. , Peter N Schlegel, M. D"— Presentation transcript:

1 Use of frozen-thawed testicular sperm for intracytoplasmic sperm injection 
Wolfgang Küpker, M.D., Ph.D., Peter N Schlegel, M.D., Safaa Al-Hasani, Ph.D., Paolo Fornara, M.D., Ph.D., Rainer Johannisson, Ph.D., Jörg Sandmann, M.D., Thilo Schill, M.D., Monika Bals-Pratsch, M.D., Michael Ludwig, M.D., Klaus Diedrich, M.D., Ph.D.  Fertility and Sterility  Volume 73, Issue 3, Pages (March 2000) DOI: /S (99)

2 Figure 1 Open testicular biopsy (two biopsy sites).
Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility  , DOI: ( /S (99) )

3 Figure 2 Extracted testicular tissue before freezing.
Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility  , DOI: ( /S (99) )

4 Figure 3 Sertoli cell–only pattern with focal spermatogenesis. Toluidine blue/pyronine stain. Original magnification, ×1,000). Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility  , DOI: ( /S (99) )

5 Figure 4 Protocol for freezing and thawing of testicular tissue.
Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility  , DOI: ( /S (99) )


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