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Taking Snapshots of Ciliary Signaling

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Presentation on theme: "Taking Snapshots of Ciliary Signaling"— Presentation transcript:

1 Taking Snapshots of Ciliary Signaling
Anna S. Nikonova, Erica A. Golemis  Developmental Cell  Volume 35, Issue 4, Pages (November 2015) DOI: /j.devcel Copyright © 2015 Elsevier Inc. Terms and Conditions

2 Figure 1 Methods Used for Assessment of Cilium-Associated Signaling Proteins Clockwise from top: cilia-APEX method described herein; TAP-tagging of ciliary or cilia-proximal proteins followed by mass spectrometry-based analysis of co-purified partners; GECI method using a Smo-mCherry-GCaMP3 genetically encoded probe to support ratiometric comparison of ciliary and cytoplasmic calcium and calcium-related signaling following laser-induced membrane rupture at the ciliary tip; biochemical studies of Chlamydomonas flagella used to generate hypotheses for studies of mammalian cilia; MudPIT of cilia purified following calcium-shock-induced release, supported by correlation profiling to known ciliary proteins. Developmental Cell  , DOI: ( /j.devcel ) Copyright © 2015 Elsevier Inc. Terms and Conditions

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