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S1PR5 is essential for human natural killer cell migration toward sphingosine-1 phosphate  Annabelle Drouillard, MSc, Anne-Laure Mathieu, PhD, Antoine.

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Presentation on theme: "S1PR5 is essential for human natural killer cell migration toward sphingosine-1 phosphate  Annabelle Drouillard, MSc, Anne-Laure Mathieu, PhD, Antoine."— Presentation transcript:

1 S1PR5 is essential for human natural killer cell migration toward sphingosine-1 phosphate 
Annabelle Drouillard, MSc, Anne-Laure Mathieu, PhD, Antoine Marçais, PhD, Alexandre Belot, MD, PhD, Sébastien Viel, PharmD, PhD, Michaël Mingueneau, PhD, Kevin Guckian, PhD, Thierry Walzer, PhD  Journal of Allergy and Clinical Immunology  Volume 141, Issue 6, Pages e1 (June 2018) DOI: /j.jaci Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Mature NK cells from human lymphoid organs are responsive to S1P and express high levels of S1PR5. A, Chemotaxis assays of blood, BM, and tonsil lymphocytes toward S1P. Panels show migration of mature NK cells (CD56+ CD57+) or naive T cells (CD3+ CCR7+ CD45RA+). N ≥ 3 donors. B, Chemotaxis assays of human BM NK-cell subsets. N = 3 donors. C, Tonsil NK-cell subsets were sorted by flow cytometry. S1PR expression was measured by RT-quantitative PCR. N = 3 sorts (3 donors). D, Chemotaxis assays of mature tonsil NK or naive T cells. Tonsil lymphocytes were incubated with compounds at the indicated concentration (in nM) for 2 hours, in the upper chamber. S1P at optimal concentration (26 nM) was then added to the lower chamber and cells were allowed to migrate. Results show the migration relative to the control condition (no inhibitor). N = 3 experiments. ns, Not significant. *P < .05; **P < .01. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 BIO blocks mouse NK-cell BM egress in vivo and human NK-cell migration toward S1P in vitro. A, Chemotaxis assays of mouse spleen NK cells or T cells toward S1P ± FTY720 or BIO Results show migration relative to control condition (no inhibitor). N = 3 experiments. **P < .01; ***P < .001. B and C, Mice were injected intravenously daily for 10 days with BIO or vehicle. B, The percentage of NK, mature NK (CD11b+CD27−), or B cells (CD19+) was measured. BM cell number was unchanged by treatments. C, Percentage of BM sinusoid mature NK and B cells labeled with intravenously injected anti-CD45. Each dot corresponds to 1 mouse. *P < .05; **P < .01. Representative of 3. D, Chemotaxis assay of human BM or tonsil lymphocytes toward S1P in presence or absence of BIO Results show migration of mature NK or naive T cells relative to control condition (no inhibitor). N = 3 experiments. *P < .05; **P < .01. Journal of Allergy and Clinical Immunology  , e1DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions


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