1. A device for in vivo 2-photon imaging of synapses in mobile mice
Advisor
Mitch Tyler
Client
Giulio Tononi
Team Members
Mark Reagan, David Leinweber, Jon Seaton, Jay Sekhon

2. Background Design Problem Client Requirements Similar Products
Outline
Background
Design Problem
Client Requirements
Similar Products
Design Phases
Phase 1 (Ball)
Phase 2 (Frame and Head device)
Testing
Prototype Design
Phase 1
Future Work

3. Sleep hypothesized to promote synaptic downscaling Why beneficial:
Background
Sleep hypothesized to promote synaptic downscaling
Why beneficial:
Energy metabolism
Signal-to-noise ratios
Performance

4. Test hypothesis Head restraint Adapt to microscope
Design Problem
Test hypothesis
Perform in vivo imaging of synapses in mice
Head restraint
Adapt to microscope

5. Fixed stereotaxic frame Finished as quickly as possible
Client Requirements
Fixed stereotaxic frame
Finished as quickly as possible
No electrical components
No movement Restrictions
Fit between lens and table

6. Similar Products
Figure 1,Dombeck et al.,Neuron 56:43, 2007

7. Phase 1: Design 1
Water Suspension

8. Phase 1: Design 2
Bearings in “Bowl”

9. Phase 1: Design 3
Four Large Bearings

10. Phase 1: Design Matrix
Total

11. Phase 1 Testing Testing Box design Flat Design Box design, with mice
Adjustability
Flat Design
Lubricant
Box design, with mice
Water Proofing
Traction
Based final Design off Testing

12. Prototype Design, Phase 1
Ball
Mesh, Waterproofing
Bearings
One inch bearings
graphite
Adjustability
Dowels

13. Test Phase 1 on trained mice
Future Work
Complete Phase 2
Test Dental Cement
Complete frame construction
Test Phase 1 on trained mice

14. Questions