1. Event-related potentials improve diagnosis of Alzheimer’s disease
Mirjana Babić Leko1, Magdalena Krbot Skorić2, Nataša Klepac3, Fran Borovečki3,4,
Lea Langer Horvat1, Željka Vogrinc5, Zdenko Sonicki6, Patrick R. Hof7, Goran Šimić1*
1Department of Neuroscience, Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
2Laboratory for Cognitive and Experimental Neurophysiology, University Hospital Centre Zagreb, Zagreb, Croatia
3Department of Neurology, University Hospital Center Zagreb, Zagreb, Croatia
4University of Zagreb School of Medicine, Department for Functional Genomics, Zagreb, Croatia
5Laboratory for Neurobiochemistry, Department of Laboratory Diagnostics, University Hospital Centre Zagreb
6Andrija Štampar School of Public Health, University of Zagreb School of Medicine, Zagreb, Croatia
7Fishberg Department of Neuroscience, Friedman Brain Institute, and Ronald M. Loeb Center for Alzheimer’s Disease, Icahn School of Medicine at Mount Sinai, New York, NY, USA
Correspondence to:
Croatian Science Foundation
Grant IP
( )
Croatian Institute
for Brain Research
University of Zagreb Medical School
Acknowledgments
Materials and methods
Introduction
This work was funded by the Croatian Science Foundation IP “Tau protein hyperphosphorylation, aggregation and trans-synaptic transfer in Alzheimer's disease: cerebrospinal fluid analysis and assessment of potential neuroprotective compounds“ to G.Š and by the Scientific Centre of Excellence for Basic, Clinical and Translational Neuroscience SCE-NEURO of the Croatian Institute for Brain Research, University of Zagreb Medical School, grant KK
The levels of ERPs measured by EEG are potential non-invasive biomarkers for the improvement of early diagnosis of AD. While P300 mainly refers to the brain response on the processing of sensory information, N200 refers to the brain response on stimulus differentiation. The aim of this study was to compare P300, N200 and RT time with CSF biomarkers of AD: Aβ1-42, t-tau, p-tau181, p-tau199, p-tau231, and VILIP-1.
The study included 49 AD, 28 MCI, 8 FTD, 8 VaD, and 4 HC subjects. Examinees that participated in auditory oddball paradigm had to complete two tasks: 1) In paradigm reaction time (RT), examinees had to press the button as the response on target auditory tones while in the 2) paradigm counting, examinees had to count all target auditory tones among non-target and interfering tones. CSF was taken by spinal tap and biomarker levels measured using ELISA.
Results
Comparison of RT, N200RT, P300RT, N200 counting and P300 counting values among AD, MCI and HC subjects. *p < 0.05.
Conclusion
Moderate to strong correlation was observed between ERPs (latency of P300 and N200 potential) and CSF biomarkers. P300 latency was the most successful biomarker in early detection of AD (with 90.9% sensitivity and 100% specificity).
Correlation of ERPs with CSF protein biomarkers. Dotted lines represent 95% confidence intervals (CI) for r value.