1. Isolation and Chracterization of the Human Syncytin Gene Promoter
109회 lab meeting
Isolation and Chracterization of the Human Syncytin Gene Promoter
석사 2년
박은실

2. Syncytin Trophoblast – syncytiotrophoblast - cytotrophoblast
Mi et al, induction of syncytialization in these cells can be prevented by syncytin antiserum.
Frendo et al, cell fusion and differentiation of normal human trophoblast cells in culture are markedly inhibited by specific syncytin antisense oligonucleotides.

3. HERV-W env & syncytin
Intact HERV-W on chromosome 7q21-7q22 have 100% identity to SYNCYTIN gene
HERV-W is highly expressed in human placenta, and weaker expression is noted in human testes. Expression is not observed in other human tissues.
little is known about the mechanisms regulating the transcription of the syncytin gene.

5. Nucleotide sequences of 5’-flanking region of HERV-W0

6. Schematic diagram of the human BAC clone RG083m05 from 7q21 to 7q22.
2073bp
26725
28795
TATA
+51~+70
Complemetary PCR
Poly(a) RNA
Mung Bean nuclease Protection Analysis

7. Transcription initiation site
30% decrease
50% decrease
90% decreasse
Deletion analysis of the 59-flanking region of the HERV-W promoter
in normal trophoblast cells.

8. DNAse 1 footprinting

9. DNase I footprint analysis of the proximal promoter region of the syncytin gene.

10. Site-Directed Mutagenesis

11. Gel shift and supershift assay

12. Gel shift and supershift analysis of FP2

13. Discussion
Although full-length elements of HERVs are noninfectious, noninfectious, they have retained functional transcriptional elements, such as promoter and enhancer sequences, within
their LTRs
Sequence analysis of the 5’-flanking region of the HERV-W gene revealed a putative CCAAT box and many putative cis-acting elements
the transcriptional regulation of the syncytin gene in trophoblast is highly dependent on the CCAAT motif and Oct site
clearly demonstrate striking differences between the 3’-LTR and the 5’-LTR.