1. Enzymes (Page 579) Enzymes are Biological Catalysts
Enzymes operate throughout the body and its various organs, e.g. brain, spinal cord, etc.
However, the majority of enzymes operate within the digestive system.
Enzymes are large protein molecules with complex three-dimensional structures. Within that structure, is a specific area called the active site.

2. Enzymes in the digestive system will break down foods to obtain the nutritive value. The substance they attack is called the substrate.
Most digestive enzymes operate by the “lock-and-key” method.
The substrate must fit and bind perfectly into the ‘active site’ in order for the enzyme to break it apart.

3. 16.6 Enzyme Action (Page 580)

4. Names of Enzymes The name of an enzyme usually ends in –ase.
identifies the reacting substance. For example,
sucrase catalyzes the reaction of sucrose.
describes the function of the enzyme. For example, oxidases catalyze oxidation.
could be a common name, particularly for the digestion enzymes, such as pepsin and trypsin.

5. Active Site The active site
is a region within an enzyme that fits the shape of the reacting molecule called a substrate.
contains amino acid R groups that bind the substrate.
releases products when the reaction is complete.

6. Enzyme-Catalyzed Reaction
In an enzyme-catalyzed
reaction
a substrate attaches to the active site.
an enzyme-substrate (ES) complex forms.
reaction occurs and products are released.
an enzyme is used over and over.
E + S ES E + P

7. Copyright © 2009 by Pearson Education, Inc.
Lock-and-Key Model
In the lock-and-key model, the
active site has a rigid shape.
enzyme only binds substrates that exactly fit the active site.
enzyme is analogous to a lock.
substrate is the key that fits that lock.
Copyright © by Pearson Education, Inc.

8. Example of an Enzyme-Catalyzed Reaction

9. Page 584
Factors affecting enzyme activity:
TEMPERATURE
Enzymes
are most active at an optimum temperature (usually 37 °C in humans).
show little activity at low temperatures.
lose activity at high temperatures as denaturation occurs.

10. pH
Enzymes in
the body have an optimum pH which will vary according to where in the body they work.
certain organs operate at lower and higher optimum pH values.

11. Substrate Concentration
As substrate concentration increases,
the rate of reaction increases (at constant enzyme concentration).
No Inhibitors
Inhibitors are molecules that cause a loss of catalytic activity.
Inhibitors will prevent an enzyme from doing its job.

12. Competitive Inhibition
See Page 586
Competitive Inhibition
A competitive inhibitor
has a structure that is similar to that of the substrate
competes with the substrate for the active site
has its effect reversed by increasing substrate concentration

13. Noncompetitive Inhibition
See Page 587
Noncompetitive Inhibition
A noncompetitive inhibitor
has a structure that is much different than the substrate
distorts the shape of the enzyme, which alters the shape of the active site
prevents the binding of the substrate
cannot have its effect reversed by adding more substrate

14. A “cofactor” is a substance which can help an enzyme to function properly.
16.8 Enzyme Cofactors
A cofactor can either be a metal ion or another enzyme.

15. Metal Ions as Coenzymes

16. Function of Coenzymes Cofactors assist enzymes in catalytic activity
may be metal ions that are bonded to one of the amino acid side chains or small organic molecules known as coenzymes

17. See Page 590 for example of Co-Enzyme activity