1. By: Zainab Gbadamosi Fall 2016
PHAGE: ZAINNY
By: Zainab Gbadamosi Fall 2016

2. SOIL COLLECTION
GPS: ,
Physical Description: Loamy enrich soil under a plant tree
Air Temperature:80^oc
Collection Date:

3. CULTURAL ENRICHMENT PLATING

4. FIRST ROUND OF PURIFICATION

5. SECOND ROUND OF PURIFICATION

6. ISOLATION TECHNIQUE Isolation technique: Cultural
Enrichment Collected from 3 rounds
of purification.
_HTL titer: 1.38 x 10^10 pfu/ml

7. WEB PLATES

8. DNA CONCERNTRATION
High concentration: ng/ul

9. DNA GEL Zainab 1 = Uncut DNA Zainab2= Clal Zainab 3= EcoRI
Zainab 4= Hindiii

11. HOST RANGE PLATE

12. HOST RANGE PLATE

13. Graph of phage vs. # bacteria infected
Zainny infected 4 Bacillus
strains