1. Proline supplementation during P5CS protein knockdown suppressed GCN2 activation.
Proline supplementation during P5CS protein knockdown suppressed GCN2 activation. Melanoma cell lines UACC 903, 1205 Lu, and A375M were transfected with siRNA-targeting ALDH18A1 or a scrambled control siRNA. Cells were maintained in 1.25 mmol/L (UACC 903, A375M) or 2.5 mmol/L (1205 Lu) proline media for 48 or 96 hours before collecting protein lysates. Western blotting compared phosphorylated with total protein levels. Protein band intensity was quantified using ImageJ software, normalized to ERK2 loading control, and expressed as a percentage of the scrambled siRNA blot intensity.
Gregory R. Kardos et al. Mol Cancer Res 2015;13:
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