Presentation is loading. Please wait.

Presentation is loading. Please wait.

Inflammation in common variable immunodeficiency is associated with a distinct CD8+ response to cytomegalovirus  Sayed Mahdi Marashi, PhD, Mohammad Raeiszadeh,

Published byJacob Meyer Modified over 6 years ago

Similar presentations

Presentation on theme: "Inflammation in common variable immunodeficiency is associated with a distinct CD8+ response to cytomegalovirus  Sayed Mahdi Marashi, PhD, Mohammad Raeiszadeh,"— Presentation transcript:

1 Inflammation in common variable immunodeficiency is associated with a distinct CD8+ response to cytomegalovirus  Sayed Mahdi Marashi, PhD, Mohammad Raeiszadeh, MSc, Sarita Workman, MSc, Afsar Rahbar, PhD, Cecilia Soderberg-Naucler, PhD, Paul Klenerman, PhD, Ronnie Chee, MBBS, A. David Webster, MD, Richard S.B. Milne, PhD, Vincent C. Emery, PhD  Journal of Allergy and Clinical Immunology  Volume 127, Issue 6, Pages e4 (June 2011) DOI: /j.jaci Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 A, Percentage of CMV (NLV) or EBV (GLC) pentamer+ cells in patients with CVID or healthy controls. B and D, Data from A showing inflammatory and noninflammatory groups. C, Frequency of CMV (NLV) pentamer+ cells in serial samples from 4 patients (Pt). Lines in A, B, and D indicate means. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Phenotyping of CMV NLV–specific CD8+ T cells. A and B, PD-1 (median fluorescence intensity [MFI]). C and D, Ki-67. E and F, HLA-DR. G and H, CD73. Bars indicate mean percentage values. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Proliferation of CMV-specific CD8+ T cells. A, Representative fluorescence-activated cell sorting plots. B and C, Analysis of inflammatory (n = 6) and noninflammatory (n = 8) samples. Insert in B shows generational analysis after NLV stimulation. D, Mixed peptide stimulation. E, Specificity (CMV, black; EBV, white) of expanded cells from D. Data obtained 5 days after stimulation. PHA, Phytohemagglutinin; Stim, stimulation. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Immunohistochemical detection of CMV antigens. Colon tissue from representative patient with inflammatory CVID (A, B, E, F, I, J) or healthy control subject (C, D, G, H, K, L) was stained for CMV IEA (A, C, E, G) or LA (B, D, F, H); α-actin (I, K) or processed without primary antibody (J, L). E, F, G, and H are enlargements of areas boxed in A, B, C, and D, respectively. Brown staining (diaminobenzidine chromogen) indicates presence of antigen. Original magnification ×40 (A and B); ×20 (C, D, I-L). Bars are 50 μm. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E1 Cytokine production and degranulation. A, Proportion of responding CD8+ T cells producing IFN-γ, TNF-α, or both after pp65 peptide pool stimulation. (Means from 19 patients with CVID and 12 healthy controls.) B, Frequency of double producers (DP) among total CD8+ cells. C, Percentage of double producers that degranulated. Bars in B and C indicate means. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E2 Phenotyping of CMV NLV–specific CD8+ T cells. A and B, PD-1 (frequency). C, Representative fluorescence-activated cell sorting plots for anti–Ki-67 and isotype control staining on CMV NLV pentamer+ CD8+ T cells. D and E, CD57 (frequency). Bars in A, B, D, and E indicate mean percentage values. PE, Phycoerythrin. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E3 High-avidity CMV NLV–specific CD8+ cells in inflammatory CVID. A and C, Representative fluorescence-activated cell sorting plots of normal and null tetramer staining for an inflammatory (A) and a noninflammatory patient (C). B and D, Bar graphs showing the percentage of total CD8+ T cells recognized by the normal (black) and null (white) tetramers in patients with inflammatory (B) and noninflammatory (D) CVID. Journal of Allergy and Clinical Immunology  , e4DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Inflammation in common variable immunodeficiency is associated with a distinct CD8+ response to cytomegalovirus  Sayed Mahdi Marashi, PhD, Mohammad Raeiszadeh,"

Similar presentations

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 
Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy  Erik Wambre, PhD, Jonathan.

Differentiation stage determines pathologic and protective allergen-specific CD4+ T-cell outcomes during specific immunotherapy  Erik Wambre, PhD, Jonathan.
Specific immunotherapy modifies allergen-specific CD4+ T-cell responses in an epitope- dependent manner  Erik Wambre, PhD, Jonathan H. DeLong, BSc, Eddie.

Specific immunotherapy modifies allergen-specific CD4+ T-cell responses in an epitope- dependent manner  Erik Wambre, PhD, Jonathan H. DeLong, BSc, Eddie.
Comparison of the allergic and nonallergic CD4+ T-cell responses to the major dog allergen Can f 1  Suvi Parviainen, MSc, Antti Taivainen, MD, PhD, Aino.

Comparison of the allergic and nonallergic CD4+ T-cell responses to the major dog allergen Can f 1  Suvi Parviainen, MSc, Antti Taivainen, MD, PhD, Aino.
The loss of IgM memory B cells correlates with clinical disease in common variable immunodeficiency  Rita Carsetti, MD, Maria Manuela Rosado, PhD, Simona.

The loss of IgM memory B cells correlates with clinical disease in common variable immunodeficiency  Rita Carsetti, MD, Maria Manuela Rosado, PhD, Simona.
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Human slan (6-sulfo LacNAc) dendritic cells are inflammatory dermal dendritic cells in psoriasis and drive strong Th17/Th1 T-cell responses  Anja Hänsel,

Human slan (6-sulfo LacNAc) dendritic cells are inflammatory dermal dendritic cells in psoriasis and drive strong Th17/Th1 T-cell responses  Anja Hänsel,
Combined immunodeficiency due to JAK3 mutation in a child presenting with skin granuloma  Alessia Scarselli, MD, Silvia Di Cesare, BSc, Gigliola Di Matteo,

Combined immunodeficiency due to JAK3 mutation in a child presenting with skin granuloma  Alessia Scarselli, MD, Silvia Di Cesare, BSc, Gigliola Di Matteo,
Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 
Obstructive sleep apnea syndrome and inflammation

Obstructive sleep apnea syndrome and inflammation
Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.
Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy 
IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,

IgE cross-linking impairs monocyte antiviral responses and inhibits influenza-driven TH1 differentiation  Regina K. Rowe, MD, PhD, David M. Pyle, MD,
Human B cells promote T-cell plasticity to optimize antibody response by inducing coexpression of TH1/TFH signatures  Jelle de Wit, PhD, Tineke Jorritsma,

Human B cells promote T-cell plasticity to optimize antibody response by inducing coexpression of TH1/TFH signatures  Jelle de Wit, PhD, Tineke Jorritsma,
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
CD4+ T cells in patients with chronic inflammatory rheumatic disorders show distinct levels of exhaustion  Theresa Frenz, PhD, Elena Grabski, PhD, Daniela.

CD4+ T cells in patients with chronic inflammatory rheumatic disorders show distinct levels of exhaustion  Theresa Frenz, PhD, Elena Grabski, PhD, Daniela.
Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production  Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,
Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,

Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,
Protein microarrays identify disease-specific anti-cytokine autoantibody profiles in the landscape of immunodeficiency  Jacob M. Rosenberg, ScB, Jordan.

Protein microarrays identify disease-specific anti-cytokine autoantibody profiles in the landscape of immunodeficiency  Jacob M. Rosenberg, ScB, Jordan.
Identification of a subset of human natural killer cells expressing high levels of programmed death 1: A phenotypic and functional characterization  Silvia.

Identification of a subset of human natural killer cells expressing high levels of programmed death 1: A phenotypic and functional characterization  Silvia.

Similar presentations

Ads by Google