1. Volume 141, Issue 3, Pages 972-981.e2 (September 2011)
TLE1 Modifies the Effects of NOD2 in the Pathogenesis of Crohn's Disease 
Elaine R. Nimmo, Craig Stevens, Anne M. Phillips, Amanda Smith, Hazel E. Drummond, Colin L. Noble, Michael Quail, Gail Davies, Marian C. Aldhous, David C. Wilson, Jack Satsangi 
Gastroenterology 
Volume 141, Issue 3, Pages e2 (September 2011)
DOI: /j.gastro
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2. Figure 1
Cells (SW480 or Cos7) were transfected with a NOD2–Myc construct and harvested 24 hours later. NOD2–Myc was co-immunoprecipitated using antibodies against the Y2H proteins, (A) TLE1, (B) PPP2R5E, (C) FIS1, (D) HTATIP, (E) Vimentin, and (F) GALNT2, and protein was used in Western blots. Blots were probed with anti-Myc antibody to detect the NOD2–Myc fusion protein. Where gel lanes have been removed digitally this is indicated by dotted lines. In all cases shown, a NOD2–Myc band of the expected size was observed.
Gastroenterology  , e2DOI: ( /j.gastro )
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3. Figure 2
(A) HEK293 cells were transfected for 24 hours with FLAG–TLE1 or FLAG–EMPTY vector as indicated. After transfection, cell extracts were prepared and resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. Exogenous TLE1 was detected by immunoblotting with anti-FLAG antibodies. The dotted line indicates that nonrelevant lanes were removed from the image. (B) HEK293 cells seeded onto glass coverslips were co-transfected with HA-NOD2 and FLAG–TLE1. After transfection, cells were fixed, permeabilized, and co-immunostained for exogenous NOD2 (red) with anti–HA-11 antibodies and exogenous TLE1 (green) with anti-FLAG antibodies. Areas where NOD2 and TLE1 co-localize (yellow) are highlighted. (C) HEK293 cells were transfected with HA–NOD2 (50 ng) together with FLAG–TLE1 (100, 200, 300, 400, or 500 ng), NF-κB–luciferase (200 ng), and the internal control renilla (100 ng). The total amount of DNA transfected was balanced with FLAG empty vector. After 24 hours, cells were harvested and the indicated data were derived from triplicate readings. Results are reported as the mean ± standard deviation (***P < .0001, n = 3), with P values calculated using an unpaired t test in Graphpad v4.03.
Gastroenterology  , e2DOI: ( /j.gastro )
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4. Figure 3
Analysis of microarray data on 6 proteins identified in the Y2H screen. (A) TLE1, (B) PPP2R5E, (C) FIS1, (D) HTATIP, (E) Vimentin, and (F) GALNT2. Samples were separated into controls (con), CD, and UC, and inflamed (in) and uninflamed (un), and the graph of mRNA expression is shown for each. Gene expression was normalized using the Stratagene Universal Reference. If a difference in expression levels generated a P value of less than .05, it is shown. Horizontal bars indicate median levels.
Gastroenterology  , e2DOI: ( /j.gastro )
Copyright © 2011 AGA Institute Terms and Conditions

5. Supplementary Figure 1
Map of TLE1 (Ensembl NCBI36) and position of SNPs genotyped.
Gastroenterology  , e2DOI: ( /j.gastro )
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