1. ZBTB48 associates with short and long telomeres in vivo
ZBTB48 associates with short and long telomeres in vivo TTAGGG content of telomeric reads in ChIPseq samples from U2OS WT, U2OS ZBTB48 KO and U2OS HOT1 KO clones using IgG, TRF2, ZBTB48 Atlas, ZBTB48 GeneTex and HOT1 antibodies as well as FLAG antibody for FLAG‐ZBTB48 WT and point mutants for ZnF10 and ZnF11. The percentage contribution to all reads containing 1–8× TTAGGG repeats is shown. All reactions were performed in technical replicates on two independent WT and KO clones each.MEME sequence logo and bit score for the top 500 extratelomeric ChIPseq peaks for ZBTB48 Atlas, ZBTB48 GeneTex, TRF2 and HOT1 antibodies compared to IgG samples in U2OS. For each antibody, the most frequent motif is shown.TTAGGG enrichment in ChIPseq samples with reads with 7× or 8× TTAGGG repeats. Absolute read counts from ChIPseq reactions from each two HeLa and U2OS WT and KO clones with two independent antibodies for ZBTB48 and HOT1 are shown (left). The corresponding fold enrichments are calculated (right) and compared to TRF2 ChIPseq reactions for which fold enrichments are calculated relative to IgG samples. Error bars represent standard deviations (n = 4), and P‐values are based on Student's t‐test with ** indicating P < 0.01.
Arne Jahn et al. EMBO Rep. 2017;embr
© as stated in the article, figure or figure legend