Presentation is loading. Please wait.

Presentation is loading. Please wait.

Performance Assessment of Eight High-Throughput PCR Assays for Viral Load Quantitation of Oncogenic HPV Types  Roberto Flores-Munguia, Erin Siegel, Walter.

Published byDonata Barone Modified over 6 years ago

Similar presentations

Presentation on theme: "Performance Assessment of Eight High-Throughput PCR Assays for Viral Load Quantitation of Oncogenic HPV Types  Roberto Flores-Munguia, Erin Siegel, Walter."— Presentation transcript:

1 Performance Assessment of Eight High-Throughput PCR Assays for Viral Load Quantitation of Oncogenic HPV Types  Roberto Flores-Munguia, Erin Siegel, Walter T. Klimecki, Anna R. Giuliano  The Journal of Molecular Diagnostics  Volume 6, Issue 2, Pages (May 2004) DOI: /S (10) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Accuracy of HPV quantitation. Experimentally determined viral load values (y axis) for HPV-16 (A), HPV-18 (B), HPV-31 (C), HPV-39 (D), HPV-45 (E), HPV-51 (F), HPV-52 (G), and HPV-58 (H) were plotted against the expected input copy number (x axis) in a log-log scale. Each point represents the mean value with the SE for triplicate determinations of eight independent assays (n = 3 × 8). Accuracy was determined using the slope value of the tendency line (allowed error window, m = 1.0 ± 0.05). The Journal of Molecular Diagnostics 2004 6, DOI: ( /S (10) ) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Specificity assay for HPV-16 (A), HPV-18 (B), HPV-31 (C), HPV-39 (D), HPV-45 (E), HPV-51 (F), HPV-52 (G), and HPV-58 (H) using heterologous templates and type-specific fluorogenic oligo-probes. The bar (▪) in each panel corresponds to the mean viral load value for three replicates on the same assay ± SD. The Journal of Molecular Diagnostics 2004 6, DOI: ( /S (10) ) Copyright © 2004 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Download ppt "Performance Assessment of Eight High-Throughput PCR Assays for Viral Load Quantitation of Oncogenic HPV Types  Roberto Flores-Munguia, Erin Siegel, Walter."

Similar presentations

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
Nine Novel Germline Gene Variants in the RET Proto-Oncogene Identified in Twelve Unrelated Cases Syed A. Ahmed, Karen Snow-Bailey, W. Edward Highsmith,

Nine Novel Germline Gene Variants in the RET Proto-Oncogene Identified in Twelve Unrelated Cases Syed A. Ahmed, Karen Snow-Bailey, W. Edward Highsmith,
Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,

Development of a Novel One-Tube Isothermal Reverse Transcription Thermophilic Helicase-Dependent Amplification Platform for Rapid RNA Detection James Goldmeyer,
CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.
Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,

Quantification of Parvovirus B19 DNA Using COBAS AmpliPrep Automated Sample Preparation and LightCycler Real-Time PCR Stefan Schorling, Gunnar Schalasta,
Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.

Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.
Clinical Validation of a New Triplex Real-Time Polymerase Chain Reaction Assay for the Detection and Discrimination of Herpes simplex Virus Types 1 and.

Clinical Validation of a New Triplex Real-Time Polymerase Chain Reaction Assay for the Detection and Discrimination of Herpes simplex Virus Types 1 and.
Detection of Exon 12 Mutations in the JAK2 Gene

Detection of Exon 12 Mutations in the JAK2 Gene
Performance Characteristics of a Quantitative, Homogeneous TaqMan RT-PCR Test for HCV RNA  Joerg Kleiber, Thomas Walter, Gerd Haberhausen, Sue Tsang,

Performance Characteristics of a Quantitative, Homogeneous TaqMan RT-PCR Test for HCV RNA  Joerg Kleiber, Thomas Walter, Gerd Haberhausen, Sue Tsang,
Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers  Rebecca A. Betensky, Catherine.

Statistical Considerations for Immunohistochemistry Panel Development after Gene Expression Profiling of Human Cancers  Rebecca A. Betensky, Catherine.
Performance Characteristics of a Quantitative Hepatitis C Virus RNA Assay Using COBAS AmpliPrep Total Nucleic Acid Isolation and COBAS TaqMan Hepatitis.

Performance Characteristics of a Quantitative Hepatitis C Virus RNA Assay Using COBAS AmpliPrep Total Nucleic Acid Isolation and COBAS TaqMan Hepatitis.
Comparative Study and Analytical Verification of PCR Methods for the Diagnosis of Congenital Chagas Disease  Carolina I. Cura, Juan C. Ramírez, Marcelo.

Comparative Study and Analytical Verification of PCR Methods for the Diagnosis of Congenital Chagas Disease  Carolina I. Cura, Juan C. Ramírez, Marcelo.
Robustness of Amplicon Deep Sequencing Underlines Its Utility in Clinical Applications  Vera Grossmann, Andreas Roller, Hans-Ulrich Klein, Sandra Weissmann,

Robustness of Amplicon Deep Sequencing Underlines Its Utility in Clinical Applications  Vera Grossmann, Andreas Roller, Hans-Ulrich Klein, Sandra Weissmann,
Molecular Genetics of Pediatric Soft Tissue Tumors

Molecular Genetics of Pediatric Soft Tissue Tumors
Precision Profiling and Components of Variability Analysis for Affymetrix Microarray Assays Run in a Clinical Context  Thomas M. Daly, Carmen M. Dumaual,

Precision Profiling and Components of Variability Analysis for Affymetrix Microarray Assays Run in a Clinical Context  Thomas M. Daly, Carmen M. Dumaual,
High Quality Assessment of DNA Methylation in Archival Tissues from Colorectal Cancer Patients Using Quantitative High-Resolution Melting Analysis  Marija.

High Quality Assessment of DNA Methylation in Archival Tissues from Colorectal Cancer Patients Using Quantitative High-Resolution Melting Analysis  Marija.
Clinical Validation of a New Triplex Real-Time Polymerase Chain Reaction Assay for the Detection and Discrimination of Herpes simplex Virus Types 1 and.

Clinical Validation of a New Triplex Real-Time Polymerase Chain Reaction Assay for the Detection and Discrimination of Herpes simplex Virus Types 1 and.
Establishment and Study of Different Real-Time Polymerase Chain Reaction Assays for the Quantification of Cells with Deletions of Chromosome 7  Elia Mattarucchi,

Establishment and Study of Different Real-Time Polymerase Chain Reaction Assays for the Quantification of Cells with Deletions of Chromosome 7  Elia Mattarucchi,
A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia  Paul A.

A DNA Real-Time Quantitative PCR Method Suitable for Routine Monitoring of Low Levels of Minimal Residual Disease in Chronic Myeloid Leukemia  Paul A.
Mutation Screening in Juvenile Polyposis Syndrome

Mutation Screening in Juvenile Polyposis Syndrome

Similar presentations

Ads by Google